DNA binding proteins of the filamentous phages CTXphi and VGJphi of Vibrio cholerae
J Bacteriol
; 191(18)Sept. 2009. graf, ilus
Article
em En
| CUMED
| ID: cum-43990
Biblioteca responsável:
CU1.1
ABSTRACT
The native product of open reading frame 112 (orf112) and a recombinant variant of the RstB protein, encoded by Vibrio cholerae pathogen-specific bacteriophages VGJphi and CTXphi, respectively, were purified to more than 90% homogeneity. Orf112 protein was shown to specifically bind single-stranded genomic DNA of VGJphi; however, RstB protein unexpectedly bound double-stranded DNA in addition to the single-stranded genomic DNA. The DNA binding properties of these proteins may explain their requirement for the rolling circle replication of the respective phages and RstB's requirement for single-stranded-DNA chromosomal integration of CTXphi phage dependent on XerCD recombinases(AU)
Texto completo:
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Coleções:
06-national
/
CU
Base de dados:
CUMED
Assunto principal:
Vibrio cholerae
/
DNA
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Proteínas de Transporte
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Toxina da Cólera
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Charibdotoxina
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2009
Tipo de documento:
Article