Glycation pattern of captopril in diabetics

ABSTRACT

Captopril is a chemical which acts as vasodilator and angiotensin- I converting enzyme inhibitor. The present attempt is to study the glycation inhibition pattern of captopril in diabetics and normals. Experimental Study. This study was conducted at the Research Lab., Department of Biochemistry, University of Agriculture, Faisalabad from May 2006 to February 2007. The study was designed on blood plasma from apparently healthy non-diabetic and diabetic persons. Plasma samples with different concentrations of glucose and that of captopril were prepared, followed by incubation for 5 weeks at 37 °C temperature. Glucose was estimated by glucose oxidase method before and after dialysis. Glycation level was measured by TBA and Periodate assays. Increased glycation was observed from 1[st] to 3[rd] week of incubation while it decreased after 5[th] week due to the formation of advanced glycatiori end products. 10 mM concentration of captopril showed fairly good response to decrease glycation as compared to its other two concentrations. Highest concentration of captopril produced overall good enough of inhibition. Periodate borohydride appeared to be more reliable and sensitive glycation assay as compared to TBA