effects of acetazolamide on ischemia reperfused isolated hearts of 2- and 8-week-old rabbits

ABSTRACT

To investigate the effects of acetazolamide on the ischemia-reperfused isolated hearts of 2- and 8-week-old rabbits. This study was conducted at the Kermanshah Medical Biology Research Center, Kermanshah, Iran from March to September 2011. Two- [n=17] and 8-week old [n=17] rabbits were separately divided into 2 control [n=9], and test [n=8] groups. Isolated hearts were subjected to 35 minutes ischemia and 30 minutes reperfusion. Acetazolamide [100 microgr/l] was added to the perfusion solution for 10 minutes before ischemia in the test group. Cardiac parameters including ventricular pressure, heart rate [HR], and rate pressure product [RPP] were measured. Data sets were analyzed by t-test. Following acetazolamide administration the change percentage of HR was significantly different in the 2-week [91 +/- 1.1%] compared with the 8- week [96 +/- 0.8%] test groups [p=0.0016]. Recovery percentage of RPP in reperfusion was lower [p=0.005] in the 8- [28.9 +/- 3.4%] than the 2-week test groups [45.2 +/- 3.5%]. The 2-week hearts elicited more rapid response to carbonic anhydrase [CA] inhibition than the 8-week group. However, acetazolamide does not exacerbate ischemia-reperfusion [I/R] injury in the 2-week hearts. Therefore, it was revealed that after inhibition of CA, the age dependent pattern of I/ R injury was similar to that of the normal hearts. Inspite of the CA important role in the normal heart function, it is not a determining factor in I/R injury in different ages