Localisation of COX-2 protein is different in breast ductal carcinoma and adjacent non-tumour ductal epithelium
Clin. transl. oncol. (Print)
; 7(6): 239-243, jul. 2005. ilus, tab
Article
em En
| IBECS
| ID: ibc-040764
Biblioteca responsável:
ES1.1
Localização: ES1.1 - BNCS
RESUMEN
Introduction. A number of findings suggest that cyclooxygenase-2 (COX-2) is overexpressed in breast tumours. However, there is a lack of consensus in the literature regarding the pattern of expression of this protein in invasive breast ductal carcinoma and in the adjacent non-tumour ductal epithelium. This study compares the expression of COX-2 mRNA and protein in breast ductal carcinoma relative to non-tumour breast tissue. Material and methods. We analysed the expression of COX-2 mRNA by quantitative PCR, and COX-2 protein by immunohistochemistry in invasive ductal carcinoma as well as in non-tumour adjacent ductal epithelium from 34 breast biopsies diagnosed as being invasive ductal carcinoma. As control, we analysed expression of COX-2 protein by immunohistochemistry in surgically-resected benign breast lesions. Results. Our results show that COX-2 mRNA and protein are overexpressed in non-tumour ductal epithelium compared with invasive ductal carcinoma. However, the pattern of the protein expression is different in tumour and non-tumour tissue: COX-2 protein is expressed predominantly in the membrane of the non-tumour ductal epithelium (including in benign breast lesions) while, in invasive ductal carcinoma cells, it is localised in the cytoplasm. Conclusions. The non-tumour ductal epithelium adjacent to invasive ductal carcinoma shows a higher COX-2 expression than does the invasive ductal carcinoma. However, the different localisation of the immunohistochemically-detected protein suggests a possible post-translational regulation of the protein
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Coleções:
06-national
/
ES
Base de dados:
IBECS
Assunto principal:
Neoplasias da Mama
/
Biomarcadores Tumorais
/
Prostaglandina-Endoperóxido Sintases
Limite:
Female
/
Humans
Idioma:
En
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2005
Tipo de documento:
Article