Phylogenetic diversity of methyl-coenzyme Mreductase (mcrA) gene and methanogenesisfrom trimethylamine in hypersaline environments
Int. microbiol
; 15(1): 33-41, mar. 2012. ilus, tab
Artigo
em Inglês
| IBECS
| ID: ibc-102990
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Methanogens have been reported in complex microbial communities from hypersaline environments, but little is known about their phylogenetic diversity. In this work, methane concentrations in environmental gas samples were determined while methane production rates were measured in microcosm experiments with competitive and non-competitive substrates. In addition, the phylogenetic diversity of methanogens in microbial mats from two geographical locations was analyzed the well studied Guerrero Negro hypersaline ecosystem, and a site not previously investigated, namely Laguna San Ignacio, Baja California Sur, Mexico. Methanogenesis in these microbial mats was suspected based on the detection of methane (in the range of 0.00086 to 3.204 %) in environmental gas samples. Microcosm experiments confirmed methane production by the mats and demonstrated that it was promoted only by non-competitive substrates (trimethylamine and methanol), suggesting that methylotrophy is the main characteristic process by which these hypersaline microbial mats produce methane. Phylogenetic analysis of amino acid sequences of the methyl coenzyme-M reductase (mcrA) gene from natural and manipulated samples revealed various methylotrophic methanogens belonging exclusively to the family Methanosarcinaceae. Moderately halophilic microorganisms of the genus Methanohalophilus were predominant (>60 % of mcrA sequences retrieved). Slightly halophilic and marine microorganisms of the genera Methanococcoides and Methanolobus, respectively, were also identified, but in lower abundances (AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Águas Salinas
/
Methanosarcinaceae
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2012
Tipo de documento:
Artigo
Instituição/País de afiliação:
National Aeronautics and Space Administration/USA
/
Northwestern Center for Biological Research (CIBNOR)/Mexico
/
Scientific and Technological Research Institute of San Luis Potosi (IPICYT)/Mexico