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Intronic SNP rs3811647 of the human transferrin gene modulates its expression in hepatoma cells / El SNP intrónico rs3811647 del gen de la transferrina humano, modula su expresión en células hepáticas
Vaquero, M Pilar; Blanco-Rojo, R; Bayele, HK; Srai, SKS.
Afiliação
  • Vaquero, M Pilar; Spanish National Research Council (CSIC). Institute of Food Science, Technology and Nutrition (ICTAN). Department of Metabolism and Nutrition. Madrid. Spain
  • Blanco-Rojo, R; Spanish National Research Council (CSIC). Institute of Food Science, Technology and Nutrition (ICTAN). Department of Metabolism and Nutrition. Madrid. Spain
  • Bayele, HK; University College London. Department of Biochemistry and Molecular Biology. London. UK
  • Srai, SKS; University College London. Department of Biochemistry and Molecular Biology. London. UK
Nutr. hosp ; 27(6): 2142-2145, nov.-dic. 2012. ilus
Article em En | IBECS | ID: ibc-112205
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Introduction: Transferrin (Tf) exerts a crucial function in the maintenance of systemic iron homeostasis. The expression of the Tf gene is controlled by transcriptional mechanism, although little is known about genetic factors influence. Objective: To study the role of rs3811647 in Tf expression using an in-vitro assay on hepatoma cells. Design and Methods: Hep3B cells were co-transfected with constructs containing A (VarA-Tf-luc) and G (VarG-Tf-luc) variants of rs3811647, using luciferase as a surrogate reporter of Tf expression. Results: Luciferase assays showed a higher intrinsic enhancer activity (p < 0.05) in the A compared with the G variant. In silico analysis of SNP rs3811647 showed that the A allele might constitute a binding site for the transcription factor glucocorticoid receptor (GR). Conclusion: The A allele of SNP rs3811647 increases Tf expression in a manner that might underlie inter-individual variation in serum transferrin levels observed in different population groups (AU)
RESUMEN
Introducción: La transferrina (Tf) ejerce una función crucial en el mantenimiento de la homeostasis sistémica del hierro. La expresión del gen de la transferrina es controlada a nivel transcripcional, aunque la posible influencia de factores genéticos todavía se desconoce. Objetivo: Estudiar el papel del rs3811647 en la expresión de la transferrina mediante un ensayo in-vitro en células de hepatoma. Diseño y métodos: Células Hep3B fueron co-transfectadas con vectores que contenían las variantes A (VarA-Tf-luc) y G (VarG-Tf-luc) del rs3811647, utilizándose la luciferasa como marcador de la expresión del gen Tf. Resultados: Los ensayos con la luciferasa mostraron un mayor aumento de la expresión del gen Tf en presencia de la variante A comparada con la G (p < 0,05). El análisis in silico del SNP rs3811647 mostró que la presencia del alelo A puede constituir un sitio de unión del receptor de glucocorticoides (GR). Conclusión: El alelo A del SNP rs3811647 incrementa la expresión del gen Tf de modo que podría modular la variación interindividual en los niveles de transferrina sérica observados en diferentes poblaciones (AU)
Assuntos

Texto completo: 1 Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Transferrina / Polimorfismo de Nucleotídeo Único / Hepatócitos Limite: Humans Idioma: En Revista: Nutr. hosp Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Transferrina / Polimorfismo de Nucleotídeo Único / Hepatócitos Limite: Humans Idioma: En Revista: Nutr. hosp Ano de publicação: 2012 Tipo de documento: Article