High glucose induced rat aorta vascular smooth muscle cell oxidative injury: involvement of protein tyrosine nitration
J. physiol. biochem
; 67(4): 539-549, dic. 2011.
Artigo
em Inglês
| IBECS
| ID: ibc-122391
Biblioteca responsável:
ES1.1
Localização: BNCS
RESUMEN
No disponible
ABSTRACT
The alteration and further damage of vascular smooth muscle function have been implicated in the development of vascular complications and diabetes. Little is known about protein tyrosine nitration in vascular smooth muscle cell injury induced by high glucose. In this article, vascular smooth muscle cell was exposed to 30 and 40 mM high glucose for 72 h, and then the cell injury in vascular smooth muscle cell induced by high glucose was studied. It was found that high glucose stimulated vascular smooth muscle cell injury in a dose-dependent manner, including decreasing intracellular and extracellular glutathione contents, increasing malondialdehyde and intracellular reactive oxygen species content, increasing the production of nitric oxide (increased nitrite content in cell and medium), as well as increasing protein tyrosine nitration. By comparing protein tyrosine nitration induced by high glucose conditions and extrinsic factors (heminnitriteglucose oxidase system and 3-morpholinosydnonimine), it may be speculated that protein is nitrated selectively, and specific protein tyrosine nitration is involved in diabetic vascular complications (AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Proteínas Tirosina Quinases
/
Angiopatias Diabéticas
/
Hiperglicemia
/
Músculo Liso Vascular
Limite:
Animais
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2011
Tipo de documento:
Artigo
Instituição/País de afiliação:
Huazhong University of Science and Technology/Republic of China