Succession of the gut microbiota in the cockroach Blattella germanica
Int. microbiol
; 17(2): 99-109, jun. 2014. ilus, tab
Artigo
em Inglês
| IBECS
| ID: ibc-127304
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
The cockroach gut harbors a wide variety of microorganisms that, among other functions, collaborate in digestion and act as a barrier against pathogen colonization. Blattabacterium, a primary endosymbiont, lives in the fat body inside bacteriocytes and plays an important role in nitrogen recycling. Little is known about the mode of acquisition of gut bacteria or their ecological succession throughout the insect life cycle. Here we report on the bacterial taxa isolated from different developmental instars of the cockroach Blattella germanica. The bacterial load in the gut increased two orders of magnitude from the first to the second nymphal stage, coinciding with the incorporation of the majority of bacterial taxa, but remained similar thereafter. Pyrosequencing of the hypervariable regions V1-V3 of the 16S rRNA genes showed that the microbial composition differed significantly between adults and nymphs. Specifically, a succession was observed in which Fusobacterium accumulated with aging, while Bacteroides decreased. Blattabacterium was the only symbiont found in the ootheca, which makes the vertical transmission of gut bacteria an unlikely mode of acquisition. Scanning electron microscopy disclosed a rich bacterial biofilm in third instar nymphs, while filamentous structures were found exclusively in adults (AU)
RESUMEN
No disponible
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Baratas
/
Intestinos
Limite:
Animais
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2014
Tipo de documento:
Artigo
Instituição/País de afiliação:
Community of Valencia/Spain
/
University of Valencia/Spain