Proteomic analysis of pleural effusion from lung adenoncarcinoma patients by shotgun strategy
Clin. transl. oncol. (Print)
; 16(2): 153-157, feb. 2014. tab, ilus
Artigo
em Inglês
| IBECS
| ID: ibc-127718
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
PURPOSE:
To construct a protein catalogue of malignant pleural effusion from lung adenocarcinoma patients and to screen the potential candidates of biomarkers for diagnostic value in human lung adenocarcinoma.METHOD:
Five malignant pleural effusion samples of lung adenocarcinoma patients were collected from January 2009 to September. A composite sample was analyzed using shotgun strategy. Pleural effusion samples were separated by means of SDS-PAGE. Proteomic analysis was performed by 1D-LC-MS/MS, and then the proteins were identified using SEQUEST software and protein database search.RESULTS:
Among 230 unique proteins, 123 proteins were identified with higher confidence levels (at least two unique peptide sequences matched). Most of these proteins have been reported in plasma. However, there are 7 proteins, including JUP protein, suprabasin, annexin A2, transforming growth factor-beta-induced protein ig-h3 (βig-h3), V-set and immunoglobulin domain-containing protein 4 precursor, ifapsoriasin 2 and actin, cytoplasmic 1 have not been reported in serum.CONCLUSIONS:
Seven proteins may represent potential candidates of biomarkers. Annexin A2 is of special interest since it may play a role in the regulation of intercellular adhesion and cell proliferation (AU)RESUMEN
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Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Adenocarcinoma
/
Biomarcadores Tumorais
/
Derrame Pleural Maligno
/
Proteoma
/
Proteômica
/
Neoplasias Pulmonares
Tipo de estudo:
Estudo prognóstico
Limite:
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2014
Tipo de documento:
Artigo
Instituição/País de afiliação:
Huadong Hospital Affiliated to Fudan University/China