MicroRNA-21 controls hTERT via PTEN in human colorectal cancer cell proliferation
J. physiol. biochem
; 71(1): 59-68, mar. 2015. tab
Article
em En
| IBECS
| ID: ibc-133903
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Colorectal cancer is a major health problem worldwide. The aim of this study was to determine a role of microRNA-21 (miRNA-21) in colorectal cancer (CRC) and to elucidate miRNA-21 regulation ofhTERT by phosphatase and tensin homologue (PTEN). Protein and mRNA samples were extracted with 30 CRC samples and one CRC cell lines. The expression of miRNA-21, hTERT, PTEN in CRC tissues and CRC cell lines was measured by real-time fluorescent quantitative PCR, Western blotting. Cell viability was detected by MTT and cell cycle assay. In this study, we show that the expression of miRNA-21 was overexpressed in CRC tissue and CRC cell lines compared with the control group. The effects of miRNA-21 were then assessed in MTT assays through in vitro transfection with a miRNA-21 mimic or inhibitor. PTEN has been identified as a target gene ofmiRNA-21 in CRC cell lines. Moreover, Western blot and qRT-PCR analyses revealed that miRNA-21 increased the expression of human telomerase reverse transcriptase (hTERT) via thePTEN/ERK1/2. In addition, Western blot analyses confirmed that an inverse correlation betweenPTEN and hTERT levels of high miRNA-21 RNA-expressing CRC tissues and cell lines. Finally, these data indicate that miRNA-21 regulates hTERT expression via the PTEN/ERK1/2 signaling pathway, therefore controlling CRC cell line growth. MiRNA-21 may serve as a novel therapeutic target in CRC
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Coleções:
06-national
/
ES
Base de dados:
IBECS
Assunto principal:
Neoplasias Colorretais
/
MicroRNAs
Limite:
Humans
Idioma:
En
Revista:
J. physiol. biochem
Ano de publicação:
2015
Tipo de documento:
Article