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Nanocurcumin protects cardiomyoblasts H9c2 from hypoxia-induced hypertrophy and apoptosis by improving oxidative balance
Nehra, Sarita; Bhardwaj, Varun; Ganju, Lilly; Bansal, Anju; Saxena, Shweta; Saraswat, Deepika; Kalra, Namita.
Afiliação
  • Nehra, Sarita; Defence Research and Development Organization. Defence Institute of Physiology and Allied Science. Department of Experimental Biology. New Delhi. India
  • Bhardwaj, Varun; Defence Research and Development Organization. Defence Institute of Physiology and Allied Science. Department of Experimental Biology. New Delhi. India
  • Ganju, Lilly; Defence Research and Development Organization. Defence Institute of Physiology and Allied Science. Department of Experimental Biology. New Delhi. India
  • Bansal, Anju; Defence Research and Development Organization. Defence Institute of Physiology and Allied Science. Department of Experimental Biology. New Delhi. India
  • Saxena, Shweta; Defence Research and Development Organization. Defence Institute of Physiology and Allied Science. Department of Experimental Biology. New Delhi. India
  • Saraswat, Deepika; Defence Research and Development Organization. Defence Institute of Physiology and Allied Science. Department of Experimental Biology. New Delhi. India
  • Kalra, Namita; Defence Research and Development Organization. Institute of Nuclear Medicine and Allied Science. New Delhi. India
J. physiol. biochem ; 71(2): 239-251, jun. 2015.
Article em En | IBECS | ID: ibc-140532
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Hypoxia-induced cardiomyocyte hypertrophy is evident; however, the distinct molecular mechanism underlying the oxidative stress-mediated damages to cardiomyocytes remains unknown. Curcumin(diferuloylmethane) is known for anti-hypertrophic effects, but low bioavailability makes it unsuitable to exploit its pharmacological properties. We assessed the efficacy of nanotized curcumin, i.e. nanocurcumin, in ameliorating hypoxia-induced hypertrophy and apoptosis in H9c2 cardiomyoblasts and compared it to curcumin. H9c2 cardiomyoblasts were challenged with 0.5 % oxygen, for 24 h to assess hypoxia-induced oxidative damage, hypertrophy and consequent apoptosis. The molecular mechanism underlying the protective efficacy of nanocurcumin was evaluated in regulating Raf-1/Erk-1/2 apoptosis by caspase-3/-7 pathway and oxidative stress. Nanocurcumin ameliorated hypoxia-induced hypertrophy and apoptosis in H9c2 cells significantly (p ≤ 0.01), by downregulating atrial natriuretic factor expression, caspase-3/-7 activation, oxidative stress and stabilizing hypoxia-inducible factor-1alfa (HIF-1alfa) better than curcumin. Nanocurcumin provides insight into its use as a potential candidate in curing hypoxia-induced cardiac pathologies by restoring oxidative balance
Assuntos
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Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Fator Natriurético Atrial / Curcumina / Mioblastos Cardíacos Limite: Humans Idioma: En Revista: J. physiol. biochem Ano de publicação: 2015 Tipo de documento: Article
Buscar no Google
Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Fator Natriurético Atrial / Curcumina / Mioblastos Cardíacos Limite: Humans Idioma: En Revista: J. physiol. biochem Ano de publicação: 2015 Tipo de documento: Article