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Peripheral blood mononuclear cells from severe asthmatic children release lower amounts of IL-12 and IL-4 after LPS stimulation
Falcai, A; Soeiro-Pereira, PV; Kubo, CA; Aranda, CS; Solé, D; Condino-Neto, A.
Afiliação
  • Falcai, A; University CEUMA, São Luis. Laboratory of Immunology. MA. Brazil
  • Soeiro-Pereira, PV; Federal University of Maranhao. Departament of Medicine. Pinheiro. Brazil
  • Kubo, CA; University of Sao Paulo. Institute of Biomedical Sciences. Departament of Imunology. Sao Paulo. Brazil
  • Aranda, CS; University of Sao Paulo. Departament of Pediatrics. Division of Allergy, Clinical Immunology and Rheumatolgoy. Sao Paulo. Brazil
  • Solé, D; University of Sao Paulo. Departament of Pediatrics. Division of Allergy, Clinical Immunology and Rheumatolgoy. Sao Paulo. Brazil
  • Condino-Neto, A; University of Sao Paulo. Institute of Biomedical Sciences. Departament of Imunology. Sao Paulo. Brazil
Allergol. immunopatol ; 43(5): 482-486, sept.-oct. 2015. tab, graf
Artigo em Inglês | IBECS | ID: ibc-141110
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT

INTRODUCTION:

Asthma is an inflammatory disorder of the airways associated with bronchial hyperresponsiveness, airway obstruction, and increased mucus production, with a predominance of type 2 immune response (Th2). According to the hygiene hypothesis, exposure to environmental bacterial lipopolysaccharide (LPS) may induce a type 1 immune response (Th1), modulating the development of asthma.

OBJECTIVE:

In this study we investigated cytokine production by peripheral blood mononuclear cells (PBMC) from children and adolescents with severe asthma, in response to LPS stimulation in vitro. MATERIALS AND

METHODS:

26 children were selected 13 severe asthmatics and 13 healthy controls, aged between 5 and 18 years. They were evaluated through routine medical history, physical examination and lung function test to diagnose severe asthma. Allergy status was confirmed by skin prick test and specific IgE assay. We collected blood samples to analyse in vitro LPS-induced cytokines release by PBMC.

RESULTS:

PBMC from severe asthmatic children produced lower levels of IL-12p70 in basal conditions and after 12 and 24 h stimulation with LPS compared to healthy controls. PBMC from severe asthmatic children produced lower levels of IL-4 after 24 h LPS stimulation compared to healthy controls. PBMC from severe asthmatic children produced more levels IL-17 and IL-10 after stimulus with LPS compared to healthy controls. The release of IFN-γ, IL-5 and TNF-α by PBMC from severe asthmatic children was similar to healthy controls.

CONCLUSION:

Our results demonstrate that LPS directly influence the cytokine profile of PBMC in children with severe asthma. These observations may be potentially helpful in developing new treatment strategies
RESUMEN
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Assuntos
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Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Asma / Leucócitos Mononucleares / Lipopolissacarídeos / Interleucina-4 / Interferon gama / Linfotoxina-alfa / Células Th2 / Células Th1 / Interleucina-12 / Monitoramento Epidemiológico Tipo de estudo: Estudo de rastreamento Limite: Adolescente / Criança / Feminino / Humanos / Masculino País/Região como assunto: América do Sul / Brasil Idioma: Inglês Revista: Allergol. immunopatol Ano de publicação: 2015 Tipo de documento: Artigo Instituição/País de afiliação: Federal University of Maranhao/Brazil / University CEUMA, São Luis/Brazil / University of Sao Paulo/Brazil
Buscar no Google
Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Asma / Leucócitos Mononucleares / Lipopolissacarídeos / Interleucina-4 / Interferon gama / Linfotoxina-alfa / Células Th2 / Células Th1 / Interleucina-12 / Monitoramento Epidemiológico Tipo de estudo: Estudo de rastreamento Limite: Adolescente / Criança / Feminino / Humanos / Masculino País/Região como assunto: América do Sul / Brasil Idioma: Inglês Revista: Allergol. immunopatol Ano de publicação: 2015 Tipo de documento: Artigo Instituição/País de afiliação: Federal University of Maranhao/Brazil / University CEUMA, São Luis/Brazil / University of Sao Paulo/Brazil
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