Combined use of a new SNP-based assay and multilocus SSR markers to assess genetic diversity of Xylella fastidiosa subsp. pauca infecting citrus and coffee plants
Int. microbiol
; 18(1): 13-24, mar. 2015. ilus, tab
Artigo
em Inglês
| IBECS
| ID: ibc-141140
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Two haplotypes of Xylella fastidiosa subsp. pauca (Xfp) that correlated with their host of origin were identified in a collection of 90 isolates infecting citrus and coffee plants in Brazil, based on a single-nucleotide polymorphism in the gyrB sequence. A new single-nucleotide primer extension (SNuPE) protocol was designed for rapid identification of Xfp according to the host source. The protocol proved to be robust for the prediction of the Xfp host source in blind tests using DNA from cultures of the bacterium, infected plants, and insect vectors allowed to feed on Xfp-infected citrus plants. AMOVA and STRUCTURE analyses of microsatellite data separated most Xfp populations on the basis of their host source, indicating that they were genetically distinct. The combined use of the SNaPshot protocol and three previously developed multilocus SSR markers showed that two haplotypes and distinct isolates of Xfp infect citrus and coffee in Brazil and that multiple, genetically different isolates can be present in a single orchard or infect a single tree. This combined approach will be very useful in studies of the epidemiology of Xfp-induced diseases, host specificity of bacterial genotypes, the occurrence of Xfp host jumping, vector feeding habits, etc., in economically important cultivated plants or weed host reservoirs of Xfp in Brazil and elsewhere (AU)
RESUMEN
No disponible
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Zoonoses
Base de dados:
IBECS
Assunto principal:
Citrus
/
Coffea
/
Xylella
Tipo de estudo:
Ensaio clínico controlado
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2015
Tipo de documento:
Artigo
Instituição/País de afiliação:
Agrifood Campus of International Excellence/Spain
/
São Paulo University/Brazil
/
University of Córdoba/Spain