TLR expression profile of human gingival margin-derived stem progenitor cells
Med. oral patol. oral cir. bucal (Internet)
; 21(1): e30-e38, ene. 2016. ilus, tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-149422
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
BACKGROUND:
Gingival margin-derived stem/progenitor cells (G-MSCs) show remarkable periodontal regenerative potential in vivo. During regeneration, G-MSCs may interact with their inflammatory environment via toll-likereceptors (TLRs). The present study aimed to depict the G-MSCs TLRs expression profile. MATERIAL ANDMETHODS:
Cells were isolated from free gingival margins, STRO-1-immunomagnetically sorted and seeded to obtain single colony forming units (CFUs). G-MSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, CD146 and STRO-1 expression, and for multilineage differentiation potential. Following G-MSCsˇŻ incubation in basic or inflammatory medium (IL-1¦Â, IFN-¦Ă, IFN-¦Á, TNF-¦Á) a TLR expression profile was generated.RESULTS:
G-MSCs showed all stem/progenitor cellsˇŻ characteristics. In basic medium G-MSCs expressed TLRs 1, 2, 3, 4, 5, 6, 7, and 10. The inflammatory medium significantly up-regulated TLRs 1, 2, 4, 5, 7 and 10 and diminished TLR 6 (pˇÜ0.05, Wilcoxon-Signed-Ranks-Test).CONCLUSIONS:
The current study describes for the first time the distinctive TLRs expression profile of G-MSCs under uninflamed and inflamed conditions
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Receptores Toll-Like
/
Gengiva
Limite:
Humanos
Idioma:
Inglês
Revista:
Med. oral patol. oral cir. bucal (Internet)
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
Cairo University/Egypt
/
Christian Albrechts University/Germany