MGMT methylation assessment in glioblastoma: MS-MLPA versus human methylation 450K beadchip array and immunohistochemistry
Clin. transl. oncol. (Print)
; 18(4): 391-397, abr. 2016. tab, ilus, graf
Artigo
em Inglês
| IBECS
| ID: ibc-150454
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Purpose:
The MGMT gene encodes a DNA repair enzyme that counteracts with chemotherapy efficiency, specifically with alkylating agents such as temozolomide (TMZ). It is well established that MGMT methylation should be screened as a predictive marker for TMZ in glioblastoma, and we thus aimed to determine a reliable and practical diagnostic method of MGMT methylation detection. Patients andmethods:
55 glioblastomas were investigated for MGMT methylation status using methylation-specific multiplexed ligation probe amplification (MS-MLPA), illumina human methylation 450K BeadChip array (HM450 K) analysis, and compared to MGMT protein expression by immunohistochemistry (IHC) staining. The methylation status of promoter, intron and all MGMT CpG targeted sites were separately correlated to patients survival.Results:
In addition to MS-MLPA and 450 K concordance, our results showed significantly higher overall survival (OS) of patients receiving TMZ and presenting MGMT methylated promoter (mean OS = 21.5 months, p = 0.046). Including all glioblastoma cases and regardless of chemotherapy, MS-MLPA showed significant survival difference between MGMT methylated and unmethylated cases (mean OS = 13, p = 0.021).Conclusion:
We concluded that in glioblastoma, MGMT promoter methylation predicts TMZ sensitivity. This current comparative analysis leads to consider that MS-MLPA is a valuable as HM450 K array for MGMT methylation status screening (AU)RESUMEN
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Espanha
Base de dados:
IBECS
Assunto principal:
Retinoblastoma
/
O(6)-Metilguanina-DNA Metiltransferase
/
Metilação
Tipo de estudo:
Estudo diagnóstico
/
Estudo de avaliação
/
Estudo prognóstico
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
Farhat Hached University Hospital/Tunisia
/
Sahloul University Hospital/Tunisia