microRNA expression profile in human coronary smooth muscle cell-derived microparticles is a source of biomarkers / El perfil de expresión de microARN en micropartículas secretadas por células musculares lisas de arterias coronarias humanas es una fuente de biomarcadores
Clín. investig. arterioscler. (Ed. impr.)
; 28(4): 167-177, ago. 2016. ilus, graf, tab
Artigo
em Inglês
| IBECS
| ID: ibc-155197
Biblioteca responsável:
ES1.1
Localização: BNCS
RESUMEN
Introduction:
microRNA (miRNA) expression profile of extracellular vesicles is a potential tool for clinical practice. Despite the key role of vascular smooth muscle cells (VSMC) in cardiovascular pathology, there is limited information about the presence of miRNAs in microparticles secreted by this cell type, including human coronary artery smooth muscle cells (HCASMC). Here, we tested whether HCASMC-derived microparticles contain miRNAs and the value of these miRNAs as biomarkers.Methods:
HCASMC and explants from atherosclerotic or non-atherosclerotic areas were obtained from coronary arteries of patients undergoing heart transplant. Plasma samples were collected from normocholesterolemic controls (N=12) and familial hypercholesterolemia (FH) patients (N=12). Both groups were strictly matched for age, sex and cardiovascular risk factors. Microparticle (0.1-1μm) isolation and characterization was performed using standard techniques. VSMC-enriched miRNAs expression (miR-21-5p, -143-3p, -145-5p, -221-3p and -222-3p) was analyzed using RT-qPCR.Results:
Total RNA isolated from HCASMC-derived microparticles contained small RNAs, including VSMC-enriched miRNAs. Exposition of HCASMC to pathophysiological conditions, such as hypercholesterolemia, induced a decrease in the expression level of miR-143-3p and miR-222-3p in microparticles, not in cells. Expression levels of miR-222-3p were lower in circulating microparticles from FH patients compared to normocholesterolemic controls. Microparticles derived from atherosclerotic plaque areas showed a decreased level of miR-143-3p and miR-222-3p compared to non-atherosclerotic areas.Conclusions:
We demonstrated for the first time that microparticles secreted by HCASMC contain microRNAs. Hypercholesterolemia alters the microRNA profile of HCASMC-derived microparticles. The miRNA signature of HCASMC-derived microparticles is a source of cardiovascular biomarkers
Buscar no Google
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Doença da Artéria Coronariana
/
Doença das Coronárias
/
MicroRNAs
/
Micropartículas Derivadas de Células
Tipo de estudo:
Fatores de risco
Limite:
Humanos
Idioma:
Inglês
Revista:
Clín. investig. arterioscler. (Ed. impr.)
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
Biomedical Research Institute Sant Pau (IIB Sant Pau)/Spain
/
Hospital Universitario Miguel Servet/Spain