Bone marrow-derived cells are recruited by the melanoma tumor with endothelial cells contributing to tumor vasculature
Clin. transl. oncol. (Print)
; 19(1): 125-133, ene. 2017. tab, graf
Article
em En
| IBECS
| ID: ibc-159127
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Purpose. Tumor expansion is dependent on neovascularization, a process that requires sustained new vessel formation. Although the critical role of angiogenesis by endothelial sprouting in this process, controversy still prevails on whether angiogenesis involving bone marrow-derived endothelial cells, does contribute to this process. This study aims to evaluate the recruitment of bone marrow-derived cells by the melanoma tumor, including endothelial cells, and if they contribute to angiogenesis. Methods. A chimeric mouse model of GFP bone marrow was used to induce melanoma tumors derived from murine B16-F10 cell line. These tumors were evaluated for the presence of myeloid cells (CD11b), T lymphocytes (CD3, CD4 and CD8) and endothelial cells (VEGFR2 and CD31) derived from bone marrow. Results. Mice transplanted with GFP+ cells showed significant bone marrow chimerism (90.9 ± 0.87 %) when compared to the GFP transgenic mice (90.66 ± 2.1 %, p = 0.83) demonstrating successful engraftment of donor bone marrow stem/progenitor cells. Analysis of the murine melanoma tumor showed the presence of donor cells in the tumors (3.5 ± 1.7 %) and interestingly, these cells represent endothelial cells (CD31+ cells; 11.5 ± 6.85 %) and myeloid cells (CD11b+ cells; 80 ± 21 %), but also tumor-infiltrating lymphocytes (CD8+ T cells, 13.31 ± 0.2 %; CD4+ T-cells, 2.1 ± 1.2 %). Examination of the tumor endothelium by confocal microscopy suggests the presence of donor CD31+/GFP+ cells in the wall of some blood vessels. Conclusion. This study demonstrates that bone marrow-derived cells are recruited by the murine melanoma tumor, with myeloid cells and CD4 and CD8 T lymphocytes migrating as antitumor immune response, and endothelial cells participating of the tumor blood vessels formation (AU)
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Coleções:
06-national
/
ES
Base de dados:
IBECS
Assunto principal:
Medula Óssea
/
Células da Medula Óssea
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Movimento Celular
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Transplante de Medula Óssea
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Molécula-1 de Adesão Celular Endotelial a Plaquetas
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Neoplasias do Tronco Encefálico
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Modelos Animais
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Melanoma
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Neoplasias de Tecido Vascular
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2017
Tipo de documento:
Article