A two-hour procedure for determining the susceptibility of enterococci and staphylococci to antibiotics by a colourimetric method / Un procedimiento de dos horas para determinar la sensibilidad de estaficolococos y enterococos a los antibióticos en dos horas mediante un método colorimétrico

ABSTRACT

Introduction. Rapid determination of the antibiotic susceptibility test in bacteria remains a challenge for Clinical Microbiology laboratories. Methods. An improvement in the colorimetric antimicrobial susceptibility testing performed with resazurin in enterococci and staphylococci has been carried out. The design of method was performed using two collection strains, which have a known susceptibility. This procedure was then validated against standard commercial methods on 15 strains of staphylococci and 15 strains of enterococci from patients. Results. The essential agreement between the colorimetric method and commercial methods (E-test, MicroScan and VITEK2) was 100%. Conclusion. Resazurin allows us to obtain a reliable antibiotic susceptibility test in staphylococci and enterococci in less than two hours (AU)