Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium
Med. oral patol. oral cir. bucal (Internet)
; 22(5): e651-e659, sept. 2017. ilus, tab
Article
em En
| IBECS
| ID: ibc-166662
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Background:
The study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish. Material andMethods:
An in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces Control group 25-mm methacrylate dish (n = 6); Machined group titanium discs with machined surface (n = 6) and Experimental group titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours.Results:
A statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group).Conclusions:
In comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it (AU)RESUMEN
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Texto completo:
1
Coleções:
06-national
/
ES
Base de dados:
IBECS
Assunto principal:
Osteoblastos
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Calcificação Fisiológica
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Proteína Morfogenética Óssea 2
Tipo de estudo:
Observational_studies
Limite:
Humans
Idioma:
En
Revista:
Med. oral patol. oral cir. bucal (Internet)
Ano de publicação:
2017
Tipo de documento:
Article