Effects of cyclopiazonic acid and dexamethasone on serotonin-induced calcium responses in vascular smooth muscle cells
J. physiol. biochem
; 72(2): 245-253, jun. 2016. graf
Artigo
em Inglês
| IBECS
| ID: ibc-168269
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
We previously observed that sarcoendoplasmic reticulum Ca2+ ATPase (SERCA) blockade by cyclopiazonic acid (CPA) significantly potentiates serotonin (5-hydroxytryptamine (5-HT))-induced vascular contractions. Furthermore, 5-HT receptor antagonist methysergide partially inhibited CPA-potentiated 5-HT contractions. In the present study, we further investigated whether SERCA inhibition potentiates 5-HT-induced Ca2+ responses along with attenuating the receptor antagonism by store-operated Ca2+ (SOC) entry and protein kinase C (PKC)-mediated mechanisms. The effects of dexamethasone that was previously shown to induce SOC entry and enhance 5-HT responses were also tested. For this purpose, intracellular Ca2+ levels were monitored in A7r5 embryonic rat vascular smooth muscle cells by spectrofluorometry using the fluorescent indicator fura-2. The results showed that CPA, although not dexamethasone, significantly potentiated 5-HT-induced Ca2+ elevations. Ketanserin partially decreased 5-HT-induced and CPA-potentiated Ca2+ elevations whereas both PKC inhibitor D-sphingosine and SOC entry blocker 2-aminoethoxydiphenyl borate (2-APB) abolished the remaining responses. The data suggests that diminished antagonistic effect on 5-HT-induced Ca2+ elevations in the presence of SERCA inhibition is induced by SOC entry and PKC activation (AU)
RESUMEN
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Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Serotonina
/
Agonistas do Receptor de Serotonina
/
Sinalização do Cálcio
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Inibidores Enzimáticos
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ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático
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Indóis
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Músculo Liso Vascular
Limite:
Animais
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
University Ege/Turkey