Fine particulate matter potentiates type 2 diabetes development in high-fat diet-treated mice: stress response and extracellular to intracellular HSP70 ratio analysis
J. physiol. biochem
; 72(4): 643-656, dic. 2016. ilus, graf
Artigo
em Inglês
| IBECS
| ID: ibc-168372
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Exposure to fine particulate matter (PM2.5) air pollution is a risk factor for type 2 diabetes (T2DM). We argue whether the potentiating effect of PM2.5 over the development of T2DM in high-fat diet (HFD)-fed mice would be related to modification in cell stress response, particularly in antioxidant defenses and 70-kDa heat shock proteins (HSP70) status. Male mice were fed standard chow or HFD for 12 weeks and then randomly exposed to daily nasotropic instillation of PM2.5 for additional 12 weeks under the same diet schedule, divided into four groups (n = 14-15 each) Control, PM2.5, HFD, and HFD + PM2.5 were evaluated biometric and metabolic profiles of mice, and cellular stress response (antioxidant defense and HSP70 status) of metabolic tissues. Extracellular to intracellular HSP70 ratio ([eHSP72]/[iHSP70]), viz. H-index, was then calculated. HFD + PM2.5 mice presented a positive correlation between adiposity, increased body weight and glucose intolerance, and increased glucose and triacylglycerol plasma levels. Pancreas exhibited lower iHSP70 expression, accompanied by 3.7-fold increase in the plasma to pancreas [eHSP72]/[iHSP70] ratio. Exposure to PM2.5 markedly potentiated metabolic dysfunction in HFD-treated mice and promoted relevant alteration in cell stress response assessed by [eHSP72]/[iHSP70], a relevant biomarker of chronic low-grade inflammatory state and T2DM risk (AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Intolerância à Glucose
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Proteínas de Choque Térmico HSP70
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Diabetes Mellitus Tipo 2
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Proteínas de Choque Térmico HSP72
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Material Particulado
/
Obesidade
Tipo de estudo:
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
Federal University of Health Sciences of Porto Alegre (UFCSPA)/Brazil
/
Federal University of Rio Grande do Sul (UFRGS)/Brazil
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Regional University of Northwestern/Brazil