Your browser doesn't support javascript.
loading
Sinomenine ameliorates the airway remodelling, apoptosis of airway epithelial cells, and Th2 immune response in a murine model of chronic asthma
Işık, S; Karaman, M; Micili, SÇ; Çağlayan-Sözmen, Ş; Bağrıyanık, HA; Arıkan-Ayyıldız, Z; Uzuner, N; Karaman, Ö.
Afiliação
  • Işık, S; Dokuz Eylul University. Department of Pediatric Allergy and Immunology. Izmir. Turkey
  • Karaman, M; Dokuz Eylul University. Department of Microbiology. Izmir. Turkey
  • Micili, SÇ; Dokuz Eylul University. Department of Histology. Izmir. Turkey
  • Çağlayan-Sözmen, Ş; Dokuz Eylul University. Department of Pediatric Allergy and Immunology. Izmir. Turkey
  • Bağrıyanık, HA; Dokuz Eylul University. Department of Histology. Izmir. Turkey
  • Arıkan-Ayyıldız, Z; Dokuz Eylul University. Department of Pediatric Allergy and Immunology. Izmir. Turkey
  • Uzuner, N; Dokuz Eylul University. Department of Pediatric Allergy and Immunology. Izmir. Turkey
  • Karaman, Ö; Dokuz Eylul University. Department of Pediatric Allergy and Immunology. Izmir. Turkey
Allergol. immunopatol ; 46(1): 67-75, ene.-feb. 2018. tab, graf, ilus
Article em En | IBECS | ID: ibc-170789
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Background: Sinomenine (SIN), an alkaloid isolated from the root of Sinomenium acutum which has a variety of pharmacological effects, including anti-inflammation, immunosuppression and anti-angiogenesis. The present study aimed to evaluate the effects of SIN on airway remodelling, epithelial apoptosis, and T Helper (Th)-2 derived cytokine levels in a murine model of chronic asthma. Methods: Twenty-two BALB/c mice were divided into four groups; I (control), II (placebo), III, IV. Mice in groups III and IV received the SIN (100 mg/kg), and dexamethasone (1 mg/kg) respectively. Epithelium thickness, sub-epithelial smooth muscle thickness, number of mast and goblet cells of samples isolated from the lung were measured. Immunohistochemical scorings of the lung tissue for matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEG-F), transforming growth factor-beta (TGF-β), terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling (TUNEL) and cysteine-dependent aspartate-specific proteases (caspase)-3 were determined. IL-4, IL-5, IL-13, Nitric oxide in bronchoalveolar lavage fluid (BALF) and ovalbumin-specific immunoglobulin (Ig) E in serum were quantified by standard ELISA protocols. Results: The dose of 100 mg/kg SIN treatment provided beneficial effects on all of the histopathological findings of airway remodelling compared to placebo (p < 0.05). All cytokine levels in BALF and serum and immunohistochemical scores were significantly lower in 100 mg/kg SIN treated group compared to the placebo (p < 0.05). Conclusions: These findings suggested that the dose of 100 mg/kg SIN improved all histopathological changes of airway remodelling and its beneficial effects might be related to modulating Th-2 derived cytokines and the inhibition of apoptosis of airway epithelial cells (AU)
RESUMEN
No disponible
Assuntos
Buscar no Google
Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Asma / Extratos Vegetais / Células Th2 / Sinomenium / Células Epiteliais Alveolares / Remodelação das Vias Aéreas Tipo de estudo: Guideline / Prognostic_studies Limite: Animals Idioma: En Revista: Allergol. immunopatol Ano de publicação: 2018 Tipo de documento: Article
Buscar no Google
Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Asma / Extratos Vegetais / Células Th2 / Sinomenium / Células Epiteliais Alveolares / Remodelação das Vias Aéreas Tipo de estudo: Guideline / Prognostic_studies Limite: Animals Idioma: En Revista: Allergol. immunopatol Ano de publicação: 2018 Tipo de documento: Article