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CXCL12 gene silencing down-regulates metastatic potential via blockage of MAPK/PI3K/AP-1 signaling pathway in colon cancer
Ma, J; Su, H; Yu, B; Guo, T; Gong, Z; Qi, J; Zhao, X; Du, J.
Afiliação
  • Ma, J; Gansu Provincial People’s Hospital. Department of General Surgery. Lanzhou. China
  • Su, H; Gansu Provincial People’s Hospital. Department of General Surgery. Lanzhou. China
  • Yu, B; The Second People’s Hospital of Lanzhou. Department of General Surgery. Lanzhou. China
  • Guo, T; Gansu Provincial People’s Hospital. Department of General Surgery. Lanzhou. China
  • Gong, Z; Ningxia Medical University. Yinchuan. China
  • Qi, J; University of Gansu. Traditional Chinese Medicine. Lanzhou. China
  • Zhao, X; Ningxia Medical University. Yinchuan. China
  • Du, J; Ningxia Medical University. Yinchuan. China
Clin. transl. oncol. (Print) ; 20(8): 1035-1045, ago. 2018. ilus, tab, graf
Article em En | IBECS | ID: ibc-173687
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Background: To investigate the effect of CXCL12 gene silencing on proliferation,invasion, angiogenesis and the relationship of MAPK/PI3K/AP-1 signaling pathway in colon cancer cells. Methods: RT-PCR and Western-blot were used to detect the expression of CXCL12 mRNA and protein in four colon cancer cell lines. Human colon cancer cells were transfected with CXCL12 siRNA carrying by Lipofectamine 2000. The expression of CXCL12 protein was confirmed by immunoblotting. WST-1, invasion and angiogenesis assay were used to examine the effect on proliferation, invasion and angiogenesis in colon cancer cells after CXCL12 siRNA silence, respectively. The phosphorylation of MAPK/PI3K/AP-1 protein levels was detected by Western blotting in CXCL12 siRNA suppression DLD-1 cell. Results: CXCL12 mRNA and proteins were only expressed in DLD-1 colon cancer cell lines. CXCL12 siRNA were transfected into DLD-1 cells, the expression CXCL12 proteins was significantly inhibited (P < 0.01), and the proliferation, invasion and angiogenesis of DLD-1 cells were inhibited significantly (P < 0.01). CXCL12 gene silencing resulted in blockage of MAPK, PI3K and AP-1 phosphorylation by CXCL12-induced in DLD-1 colon cancer cell. Conclusion: The silencing CXCL12 gene significantly inhibits the proliferation, invasion and angiogenesis ability of some types colon carcinoma cells through down-regulation of MAPK/PI3K/AP-1 signaling pathway
RESUMEN
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Assuntos

Texto completo: 1 Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Neoplasias do Colo / Fator de Transcrição AP-1 / Quinases de Proteína Quinase Ativadas por Mitógeno / Quimiocina CXCL12 / Fosfatidilinositol 3-Quinase Limite: Humans Idioma: En Revista: Clin. transl. oncol. (Print) Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Neoplasias do Colo / Fator de Transcrição AP-1 / Quinases de Proteína Quinase Ativadas por Mitógeno / Quimiocina CXCL12 / Fosfatidilinositol 3-Quinase Limite: Humans Idioma: En Revista: Clin. transl. oncol. (Print) Ano de publicação: 2018 Tipo de documento: Article