Stromal expression of JNK1 and VDR is associated with the prognosis of esophageal squamous cell carcinoma
Clin. transl. oncol. (Print)
; 20(9): 1185-1195, sept. 2018. ilus, tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-173704
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Purpose:
Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide, and its outcome is poor. The purpose of this study was to determine the association between JNK1 and vitamin D receptor (VDR) expression and the prognosis of ESCC.Methods:
Immunohistochemical staining was conducted on ESCC tissue microarrays (362 pairs of ESCC and normal esophagus tissues). The epithelial and stromal expression levels of c-jun NH2-terminal kinase 1 (JNK1) and VDR were scored and correlated with the ESCC characteristics. Laser-capture-based quantitative RT-PCR was performed on ESCC tissues. The effects of JNK1 and VDR on ESCC cell proliferation and migration were analyzed in vitro by transient transfection, and protein changes were evaluated by immunoblotting.Results:
Both JNK1 and VDR were reduced in ESCC epithelial cells in comparison with the normal esophagus, but the expression of JNK1 and VDR in ESCC stromal tissues, not epithelial cells, was strongly associated with the survival time of ESCC patients. Functional studies showed that increased JNK1 suppressed cancer cell proliferation, mobility, and migration, which were linked to the alterations of VDR and metastasis-associated proteins.Conclusion:
JNK1 and VDR act as tumor suppressors, and their stromal expression levels are associated with prognosis in esophageal squamous cell carcinomaRESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Neoplasias Esofágicas
/
Carcinoma de Células Escamosas
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Células Estromais
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Receptores de Calcitriol
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Proteínas Quinases JNK Ativadas por Mitógeno
Tipo de estudo:
Estudo prognóstico
/
Fatores de risco
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
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Masculino
Idioma:
Inglês
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2018
Tipo de documento:
Artigo
Instituição/País de afiliação:
Affiliated Hospital of Jining Medical University/China
/
Jining Medical University/China
/
University of Illinois at Chicago/USA
/
Xinxiang Medical University/China