Evaluation of serum IgE in peach-allergic patients with systemic reaction by using recombinant Pru p 7 (gibberellin-regulated protein)
Allergol. immunopatol
; 46(5): 482-490, sept.-oct. 2018. tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-177884
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
BACKGROUND:
Lipid transfer protein (LTP) is a major fruit allergen. It has, however, recently been revealed that the systemic reaction in peach-allergic patients is related not only to LTP (Pru p 3) but also to gibberellin-regulated protein (Pru p 7). We investigated recombinant Pru p 7 (rPru p 7) for its potential use in worldwide standardization for the diagnosis of peach allergy.METHODS:
Natural Pru p 7 (nPru p 7) was purified from peach crude extract using a monoclonal antibody affinity column. Complementary DNA for Pru p 7 was cloned and expressed in Escherichia coli and Pichia pastoris. Serum immunoglobulin (Ig) E in peach-allergic patients was examined by enzyme-linked immunosorbent assay (ELISA) using nPru p 7 and rPru p 7 (E. coli product erPru p 7 and P. pastoris product prPru p 7).RESULTS:
Peach-allergic patients (n = 27) were diagnosed and categorized into oral reaction (n=10) or systemic reaction (n = 17). The nPru p 7 positivity based on serum IgE levels was 52% in the systemic-reaction group and 0% in the oral-reaction group (P<0.05). In the systemic-reaction group, there was no significant difference in reactivity between nPru p 7 and prPru p 7, but the reactivity of erPru p 7 was significantly lower than those of nPru p 7 and prPru p 7 (P < 0.05).CONCLUSIONS:
We found that prPru p 7 exhibited reactivity in ELISA comparable to that of nPru p 7 for the diagnosis of peach allergy with systemic reactionRESUMEN
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Imunoglobulina E
/
Antígenos de Plantas
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Prunus persica
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Hipersensibilidade Alimentar
Limite:
Adolescente
/
Adulto
/
Criança
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Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2018
Tipo de documento:
Artigo
Instituição/País de afiliação:
Aichi Children's Health and Medical Center/Japan
/
Fujita Health University School of Medicine/Japan
/
Fujita Health University Second Educational Hospital/Japan
/
Fujita Health University/Japan
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Hamamatsu University School of Medicine/Japan
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Kyoto College of Nutritional and Medical Science/Japan
/
Kyoto University/Japan
/
Kyoto Women's University/Japan
/
Mie National Hospital/Japan
/
Ryukoku University/Japan