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Immunohistochemical characteristics of ganglionated plexuses in the human gallbladder
Jen, Phillip YP; Al Akhrass, Fadi; Pray, Nathan; Day, Cassie; Oliveira, Lais M de; Ashby, Harty; Abdallah, Lina; Collins, Jaymie; Bosse-Joseph, Daniel.
Afiliação
  • Jen, Phillip YP; University of Pikeville. Department of Biology. Pikeville. United States of America
  • Al Akhrass, Fadi; Pikeville Medical Center. Pikeville. United States of America
  • Pray, Nathan; University of Pikeville. Department of Biology. Pikeville. United States of America
  • Day, Cassie; University of Pikeville. Department of Biology. Pikeville. United States of America
  • Oliveira, Lais M de; University of Pikeville. Department of Biology. Pikeville. United States of America
  • Ashby, Harty; Pikeville Medical Center. Pikeville. United States of America
  • Abdallah, Lina; Pikeville Medical Center. Pikeville. United States of America
  • Collins, Jaymie; University of Pikeville. Department of Biology. Pikeville. United States of America
  • Bosse-Joseph, Daniel; University of Pikeville. Department of Biology. Pikeville. United States of America
Eur. j. anat ; 24(1): 17-29, ene. 2020. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-186061
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Indirect triple immunolabelling techniques were used to identify the presence of choline acetyl-transferase (ChAT), neuronal nitric oxide synthase (nNOS), Dopamine beta-hydroxylase (DβH), neuromedin U-8 (NMU-8), and neuropeptide Y (NPY) in the ganglionated plexuses of the human gallbladder. Of all the intrinsic cholinergic neurons examined, NMU-8-immunoreactive (-IR) neurons appeared to be the most populous, followed closely by neurons containing NPY-IR. nNOS-IR neurons were often observed to coexist with ChAT, NMU, and NPY. Occasionally, these nNOS positive neurons were seen triple labeled with ChAT, NMU-8, and NPY. Results also indicate that not all nNOS and NMU-8-IR coexistent neurons express NPY immunoposi-tivity. Our findings suggest that these intrinsic neurons may be categorized into a distinct population of neurons that express both inhibitory and excita-tory capabilities.Intrinsic cholinergic neurons that were ChAT-IR displayed a spectrum of immunopositivity. Interestingly, a small subpopulation of these neurons ap-peared to be extremely weak ChAT-IR or simply ChAT-immunonegative. These occasionally obser-ved ChAT-immunonegative neurons at times ex-pressed single immunopositivity for NMU-8 or nNOS, while more frequently, these ChAT-immunonegative neurons were found to be single immunopositive, or at times, double immunopositi-ve for NMU-8-, NPY-, or nNOS-IR.Dopamine beta-hydroxylase (DβH) antibodies were used to confirm the lack of intrinsic sympat-hetic innervation in the human gallbladder. As suspected, in all the sections examined, DβH-IR neu-rons were not detected. The only indication of DβH immunopositivity was noted among delicate fibers surrounding the neurons and blood vessels within the organ
RESUMEN
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Assuntos
Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Imuno-Histoquímica / Coleta de Tecidos e Órgãos / Vesícula Biliar Limite: Adulto / Feminino / Humanos Idioma: Inglês Revista: Eur. j. anat Ano de publicação: 2020 Tipo de documento: Artigo Instituição/País de afiliação: Pikeville Medical Center/United States of America / University of Pikeville/United States of America
Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Imuno-Histoquímica / Coleta de Tecidos e Órgãos / Vesícula Biliar Limite: Adulto / Feminino / Humanos Idioma: Inglês Revista: Eur. j. anat Ano de publicação: 2020 Tipo de documento: Artigo Instituição/País de afiliação: Pikeville Medical Center/United States of America / University of Pikeville/United States of America
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