IgE-mediated allergic responses associated to Ailanthus altissima pollen using an animal model
Allergol. immunopatol
; 47(3): 272-276, mayo-jun. 2019. ilus
Artigo
em Inglês
| IBECS
| ID: ibc-186489
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Background:
Murine models have been widely used in the study of allergy as sensitized mice can produce IgE and/or IgG1in response after the injection of an antigen/adjuvant combination. Ailanthus altissima pollen (AAP) has been recently reported as an emerging aeroallergen in Iran. So far, several AAP candidate allergens by the screening of allergen-specific IgE in the sera from AAP sensitized patients in Iran.Objective:
The aim of the present study was to detect and compare the allergens eliciting an IgE response in a mouse model, and in human, using pollen extract of A. altissima and an immunoproteomics based approach.Methods:
The pollen proteins were extracted in phosphate-buffered saline (PBS). Thirty male BALB/c mice were randomly divided into two groups of AP extract sensitized and sham that respectively received AAP PBS extract and a PBS control by intraperitoneal injections at regular intervals. The optimized AAP protein extracts were analyzed using 2D-gel electrophoresis and were subsequently confronted to pooled sera of sensitized mice.Results:
Two-D gel electrophoresis of AAP extract allowed the separation of 125 protein spots distributed in a wide range of pI and molecular masses. Two-DE immunoblotting using pooled sera of sensitized mice led to the detection of 14 IgE reactive spots with molecular masses ranging from 12 to 40-42kDa.Conclusion:
The results do not correlate with our previous analyses using human AAP-sensitized sera. These findings reflect some differences in the sIgE reactivity to allergenic proteins in animal modelsRESUMEN
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Espanha
Base de dados:
IBECS
Assunto principal:
Pólen
/
Alérgenos
/
Rinite Alérgica Sazonal
/
Ailanthus
/
Antígenos de Plantas
/
Soros Imunes
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2019
Tipo de documento:
Artigo
Instituição/País de afiliação:
Ministry of Science Research and Technology/Iran
/
Tehran University of Medical Sciences/Iran