Anti-allergic function of alpha-Tocopherol is mediated by suppression of PI3K-PKB activity in mast cells in mouse model of allergic rhinitis
Allergol. immunopatol
; 48(4): 395-400, jul.-ago. 2020. ilus, tab
Artigo
em Inglês
| IBECS
| ID: ibc-199726
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
BACKGROUND:
Alpha-Tocopherol (alpha -TCP), one major form of vitamin E, has been known as a treatment for airway allergic inflammation. However, the role and mechanism of alpha-TCP in treating allergic rhinitis remains unclear.OBJECTIVE:
In this study we examined the inhibitory function of alpha-TCP in a mouse model of allergic rhinitis.METHODS:
Allergic phenotype was examined by hematoxylin and eosin staining. Total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were examined by ELISA. mRNA expression was measured by qPCR, protein levels were examined by Western Blot.RESULTS:
Histological analysis of the nasal membranes revealed that there was a significant reduction in inflammatory cells appearance in cross-sections in alpha-TCP treatment of Ovalbumin (OVA)-sensitized mice compared to OVA sensitized animals. In addition, eosinophils were significantly reduced in nasal mucosa of alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Lower total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were found in alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Furthermore, we found that the subepithelial distribution of tryptase positive mast cells was reduced in the alpha-TCP treatment of OVA-sensitized mice. More importantly, the PI3K-PKB pathway was suppressed by alpha-TCP in mast cells.CONCLUSIONS:
Our results demonstrated that α-TCP-mediated suppression of PI3K-PKB activity in mast cells is a potential mechanism of anti-allergic function of alpha-TCPRESUMEN
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Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Alfa-Tocoferol
/
Rinite Alérgica
/
Antioxidantes
Limite:
Animais
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2020
Tipo de documento:
Artigo
Instituição/País de afiliação:
Affiliated ZhangJiaGang Hospital of Soochow University/China
/
Hiangsu University Health Science Center/China
/
Wenzhou Medical University/China