25-Hydroxyvitamin D potentializes extracellular cathelicidin release from human PBMC stimulated ex vivo with either bacterial (LPS) or viral (P: IC) mimetics
J. physiol. biochem
; 78(2): 335342, May. 2022. graf
Artigo
em Inglês
| IBECS
| ID: ibc-215962
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ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Human cathelicidin refers to the cationic antimicrobial peptide hCAP18/LL-37. LL-37 is formed by cleavage of the propeptide hCAP18 coded by the CAMP gene. The active form of vitamin D, 1,25-dihydroxyvitamin D (1,25(OH)D), has been shown to induce the CAMP gene expression through promoter activation. We previously failed to demonstrate in a clinical trial that supplementation of 25-hydroxyvitamin D (25(OH)D) improves LL-37 serum levels. The aim of this work was to evaluate the impact of 25(OH)D supplementation on intracellular expression of CAMP and secretion of LL-37 in an ex vivo model using the peripheral blood mononuclear cells (PBMC). PBMC collected from healthy donors and incubated with different concentrations of 25(OH)D (0 ng/ml control (D0); 25 ng/ml deficient (D25); 75 ng/ml physiological (D75); 125 ng/ml supraphysiological (D125)) were stimulated or not with lipopolysaccharide (LPS, 100 ng/ml) or synthetic double-stranded RNA Poly (I C) (PIC, 10 µg/ml). The intracellular expressions of the CAMP gene and the hCAP18 peptide were measured respectively after 24-h and 48-h incubation periods. The concentration of LL-37 was determined in the culture medium after 48-h incubation. 25(OH)D significantly induced CAMP gene expression at 24 h with a maximum effect at a dose of D125 in either unstimulated (tenfold expression) or stimulated (LPS 100-fold expression; PIC 15-fold expression) conditions. Intracellular hCAP18 peptide was overexpressed at 48 h under unstimulated (1.5-fold, D125) and stimulated conditions, LPS (twofold, D125) and PIC (2.5-fold, D125). The secretion of LL-37 in the culture medium was significantly induced by 25(OH)D only in both stimulated (LPS and PIC) conditions in a dose-dependent manner. (AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Leucócitos Mononucleares
/
Lipopolissacarídeos
Limite:
Humanos
País/Região como assunto:
Europa
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Lebanese University/Lebanon
/
University of Clermont Auvergne/France