Effect of oxygen on the germination and culturability of Bacillus atrophaeus spores / Efecto del oxígeno sobre la germinación y cultivabilidad de las esporas de Bacillus atrophaeus
Int. microbiol
; 25(2): 353-363, May. 2022. ilus, graf
Artigo
em Inglês
| IBECS
| ID: ibc-216037
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
The effect of oxygen on the germination and culturability of aerobic Bacillus atrophaeus spores was investigated in this study. Under oxic or anoxic conditions, various nutritional and non-nutritional germinants were utilized to induce germination. Tb3+-dipicolinic acid fluorescence assay and phase-contrast microscopy were used to track the germination process. The final germination level, germination half time, and germination speed were used to define germination kinetics. Colony-forming unit enumeration was used to assess the culturability of germinated spores germinated with or without oxygen. The results show that in the absence of oxygen, the final germination level was unaffected, germination half time decreased by up to 35.0%, germination speed increased by up to 27.4%, and culturability decreased by up to 95.1%. It is suggested that oxygen affects some germinant receptor-dependent germination pathways, implying that biomolecules engaged in these pathways may be oxygen-sensitive. Furthermore, spores that have completed the germination process in either anoxic or oxic conditions may have different culturability. This research contributed to a better understanding of the fundamental mechanism of germination.(AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Oxigênio
/
Bacillus
/
Microscopia de Contraste de Fase
/
Germinação
/
Fluorescência
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
The Cancer Hospital of the University of Chinese Academy of Sciences/China
/
The Hong Kong Polytechnic University/China