In situ bioremediation of petroleum hydrocarboncontaminated soil: isolation and application of a Rhodococcus strain / Biorremediación in situ de suelos contaminados con hidrocarburos de petróleo: aislamiento y aplicación de una cepa de Rhodococcus
Int. microbiol
; 26(2): 411-421, May. 2023. ilus
Article
em En
| IBECS
| ID: ibc-220232
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Due to low consumption and high efficiency, in situ microbial remediation of petroleum hydrocarbons (PHs)-contaminated sites in in-service petrochemical enterprises has attracted more and more attention. In this study, a degrading strain was isolated from oil depotcontaminated soil with soil extract (PHs) as the sole carbon source, identified and named Rhodococcus sp. OBD-3. Strain OBD-3 exhibited wide adaptability and degradability over a wide range of temperatures (1537 °C), pH (6.09.0), and salinities (17% NaCl) to degrade 60.686.6% of PHs. Under extreme conditions (15 °C and 37% salinity), PHs were degraded by 60.6 ± 8.2% and more than 82.1% respectively. In OBD-3, the alkane monooxygenase genes alkB1 and alkB2 (GenBank accession numbers: MZ688386 and MZ688387) were found, which belonged to Rhodococcus by sequence alignment. Moreover, strain OBD-3 was used in lab scale remediation in which the contaminated soil with OBD-3 was isolated as the remediation object. The PHs were removed at 2,809 ± 597 mg/kg within 2 months, and the relative abundances of Sphingobium and Pseudomonas in soil increased more than fivefold. This study not only established a system for the isolation and identification of indigenous degrading strains that could efficiently degrade pollutants in the isolated environment but also enabled the isolated degrading strains to have potential application prospects in the in situ bioremediation of PHs-contaminated soils.(AU)
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Coleções:
06-national
/
ES
Base de dados:
IBECS
Assunto principal:
Biodegradação Ambiental
/
Rhodococcus
/
Petróleo
/
Hidrocarbonetos
Limite:
Humans
Idioma:
En
Revista:
Int. microbiol
Ano de publicação:
2023
Tipo de documento:
Article