Combination of Bifidobacterium breve and antibiotics against Clostridioides difficile: effect of the time interval of combination on antagonistic activity / Combinación de Bifidobacterium breve y antibióticos contra Clostridioides difficile: efecto del intervalo de tiempo de la combinación sobre la actividad antagonista
Int. microbiol
; 26(4): 833-840, Nov. 2023. graf
Artigo
em Inglês
| IBECS
| ID: ibc-227473
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Co-administration of probiotics and antibiotics has been used to prevent or treat primary Clostridioides difficile (pCDI), and the closer the interval between the combination, the more effective it is, but the reason behind this is unknown. In this study, the cell-free culture supernatant (CFCS) of Bifidobacterium breve YH68 was used in combination with vancomycin (VAN) and metronidazole (MTR) to treat C. difficile cells. The growth and biofilm production of C. difficile under different co-administration time interval treatments were determined by optical density and crystalline violet staining, respectively. The toxin production of C. difficile was determined by enzyme immunoassay, and the relative expressions of C. difficile virulence genes tcdA and tcdB were determined by real-time qPCR method. Meanwhile, the types and contents of organic acids in YH68-CFCS were investigated by LC-MS/MS. The results showed that YH68-CFCS in combination with VAN or MTR significantly inhibited the growth, biofilm production, and toxin production of C. difficile in the effective time interval range (012 h) but did not affect the expression level of C. difficile virulence genes. In addition, the effective antibacterial component of YH68-CFCS is lactic acid (LA).(AU)
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Espanha
Base de dados:
IBECS
Assunto principal:
Probióticos
/
Resistência a Vancomicina
/
Bifidobacterium breve
/
Metronidazol
/
Antibacterianos
Limite:
Humanos
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2023
Tipo de documento:
Artigo
Instituição/País de afiliação:
Nanjing Normal University/China