Gene expression during the development of Mycobacterium smegmatis biofilms on hydroxyapatite surfaces / Expresión génica durante el desarrollo de biopelículas de Mycobacterium smegmatis en superficies de hidroxiapatita
Int. microbiol
; 27(1): 257-263, Feb. 2024. graf
Artigo
em Inglês
| IBECS
| ID: ibc-230258
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Bacterial biofilms are a consortium of bacteria that are strongly bound to each other and the surface on which they developed irreversibly. Bacteria can survive adverse environmental conditions and undergo changes when transitioning from a planktonic form to community cells. The process of mycobacteria adhesion is complex, involving characteristics and properties of bacteria, surfaces, and environmental factors; therefore, the formation of different biofilms is possible. Cell wall-, lipid-, and lipid transporter-related genes (glycopeptidolipids, GroEL1, protein kinase) are important in mycobacterial biofilm development. We investigated gene expression during in vitro development of Mycobacterium smegmatis biofilms on a hydroxyapatite (HAP) surface. Biofilm formation by M. smegmatis cells was induced for 1, 2, 3, and 5 days on the HAP surface. Mycobacteria on polystyrene generated an airliquid interface biofilm, and on the fifth day, it increased by 35% in the presence of HAP. Six genes with key roles in biofilm formation were analyzed by real-time RT‒qPCR during the biofilm formation of M. smegmatis on both abiotic surfaces. The expression of groEL1, lsr2, mmpL11, mps, pknF, and rpoZ genes during biofilm formation on the HAP surface did not exhibit significant changes compared to the polystyrene surface. These genes involved in biofilm formation are not affected by HAP.(AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Proteínas de Bactérias
/
Expressão Gênica
/
Durapatita
/
Biofilmes
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Mycobacterium smegmatis
/
Hidroxiapatitas
Limite:
Humanos
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2024
Tipo de documento:
Artigo
Instituição/País de afiliação:
Centro de Investigación en Alimentación y Desarrollo/México
/
Universidad de Sonora/México