Caffeine reduces TNFalpha up-regulation in human adipose tissue primary culture

ABSTRACT

Adipose tissue secretions play an important role in the development of obesityrelatedpathologies such as diabetes. Through inflammatory cytokines production,adipose tissue stromavascular fraction cells (SVF), and essentially macrophages, promoteadipocyte insulin resistance by a paracrine way. Since xanthine family compoundssuch as caffeine were shown to decrease inflammatory production by humanblood cells, we investigated the possible effect of caffeine on Tumor Necrosis Factoralpha (TNFalpha) and Interleukin-6 (IL-6) expression by human adipose tissue primary culture.For that purpose, human subcutaneous adipose tissue obtained from healthynon-obese women (BMI 26.7 ± 2.2 kg/m2) after abdominal dermolipectomy, wassplit into explants and cultured for 6 hours with or without caffeine. Three differentconcentrations of caffeine were tested (0.5ìg/mL, 5ìg/mL and 50ìg/mL). After 6hours of treatment, explants were subjected to collagenase digestion in order to isolateadipocytes and SVF cells. Then, TNFalpha and IL-6 mRNA were analysed by realtimePCR alternatively in adipocytes and SVF cells. In parallel, we checked geneexpression of markers involved in adipocyte differenciation and in SVF cells inflammationand proliferation. Our findings show a strong and dose dependent down-regulationof TNF-alpha gene expression in both adipocyte and SVF cells whereas IL-6 wasonly down regulated in SVF cells. No effect of caffeine was noticed on the othergenes studied. Thus, caffeine, by decreasing TNFá expression, could improve adiposetissue inflammation during obesity (AU)

RESUMEN

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