Differential toxicity of antifungal protein AFP against mutants of Fusarium oxysporum
Int. microbiol
; 12(2): 115-121, jun. 2009. ilus, tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-72370
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Antifungal protein (AFP) from Aspergillus giganteus was assayed for toxicity against the Fusarium oxysporum wild-type strain and mutants in genes involved in cell signaling (DeltapacC, pacCc Deltafmk1) or cell-wall biogenesis (DeltachsV, Deltachs7, Deltagas1). The mutants were classified into two groups according to their sensitivity to AFP DeltapacC, Deltagas1 and Deltachs7, which were significantly more resistant to AFP than the wild-type, and pacCC, Deltafmk1 and DeltachsV, which were more sensitive. Western blot analysis revealed increased binding of AFP to the three resistant mutants, DeltapacC, Deltagas1 and Deltachs7, but also to DeltachsV, indicating that differential binding may not be a key determinant for sensitivity. Addition of Ca2+ or K+ dramatically reduced antifungal activity and binding of AFP, suggesting that these cations compete for the same targets as AFP at the surface of the fungal cell (AU)
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Espanha
Base de dados:
IBECS
Assunto principal:
Aspergillus
/
Fusarium
/
Antifúngicos
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2009
Tipo de documento:
Artigo
Instituição/País de afiliação:
Complutense University of Madrid/Spain
/
University of Córdoba/Spain