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FISH and Calcofluor staining techniques to detect in situ filamentous fungal biofilms in water
Gonçalves, AB; Santos, IM; Paterson, RR; Lima, N.
Afiliação
  • Gonçalves, AB; Universidade do Minho. Centro de Engenharia Biológica, and Micoteca. Braga. Portugal
  • Santos, IM; Universidade do Minho. Centro de Engenharia Biológica, and Micoteca. Braga. Portugal
  • Paterson, RR; Universidade do Minho. Centro de Engenharia Biológica, and Micoteca. Braga. Portugal
  • Lima, N; Universidade do Minho. Centro de Engenharia Biológica, and Micoteca. Braga. Portugal
Rev. iberoam. micol ; 23(3): 194-198, sept. 2006. tab, ilus
Article em En | IBECS | ID: ibc-75390
Biblioteca responsável: ES1.1
Localização: BNCS
ABSTRACT
Filamentous fungi are a ubiquitous and diverse group of eukaryotic organisms and may contribute, along with bacteria, yeasts, protozoa and viruses, to the formation of biofilms in water distribution systems. However, fungal involvement in biofilms has not been demonstrated unambiguously. Furthermore, these fungi may be responsible for the production of tastes, odours and mycotoxins in drinking water making their early detection important. The detection of fme these problems a combination of two fluorescent techniques for direct detection was tested: (a) Fluorescence In Situ Hybridization (FISH) employing the universal rRNA probe EUK516, labelled with the red Cy3, followed by (b) staining with Calcofluor White MR2 fluorescent dye which stains fungal cell walls blue. Pure cultures of Penicillium brevicompactum were used to establish the methods followed by separate experiments with real water biofilm samples in PVC-C and cast iron coupons. FISH demonstrated eukaryotic microrganisms after approximately 5 h while the calcofluor method revealed chitinous filamentous structures in less than one hour. When the two methods were combined, additional resolution was obtained from the images of filamentous walls (blue) with intact protoplasm (red). In conclusion, FISH and Calcofluor staining provide rapid, direct and unambiguous information on the involvement of ff in biofilms which form in water(AU)
Assuntos
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Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Penicillium / Microbiologia da Água / Benzenossulfonatos / Hibridização in Situ Fluorescente / Biofilmes / Corantes Fluorescentes Tipo de estudo: Evaluation_studies / Screening_studies Idioma: En Revista: Rev. iberoam. micol Ano de publicação: 2006 Tipo de documento: Article
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Coleções: 06-national / ES Base de dados: IBECS Assunto principal: Penicillium / Microbiologia da Água / Benzenossulfonatos / Hibridização in Situ Fluorescente / Biofilmes / Corantes Fluorescentes Tipo de estudo: Evaluation_studies / Screening_studies Idioma: En Revista: Rev. iberoam. micol Ano de publicação: 2006 Tipo de documento: Article