Protein expression changed by nicotinein rat vascular smooth muscle cells

ABSTRACT

In order to observe the effects of nicotine on protein expression in rat vascularsmooth muscle cells (SMCs), nicotine treated SMCs were studied by proteomic technologiescombining two-dimensional electrophoresis (2-DE) and peptide mass fingerprinting(PMF). Real-time RT–PCR was used to validate the differentiallyexpressed proteins. We found that 11 protein spots were significantly up-regulatedand one down-regulated by nicotine treatment. The results of PMF showed thatthese up- and down-regulated proteins could be divided into three groups accordingto their functions cytoskeleton proteins, regulatory proteins and enzymes. Simultaneously,we also verified their consistent alteration at the transcriptional levelthrough real-time RT–PCR. The affected proteins turned out to be mainly associatedwith cell migration, proliferation and energy metabolism, and are responsible fornicotine-related cardiovascular damage (AU)

RESUMEN

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