Application of stained blood cells forthe differentiation of arteriesand nerves in cadavers

ABSTRACT

The demonstration of the functional arteriesto first year medical students in the dissectionroom is very important, but after cadaver fixationwith formalin and other preservingmaterials the arteries will be empty and thefixative material will take the place of blood.Thus, the arteries will be colorless and studentswill find it hard to differentiate betweenarteries and nerves. The aim of this study wasto stain arteries and differentiate them fromnerves in cadavers by means of an easymethod, the use of stained blood cells.Whole blood cells (packed cells) were initiallytreated with a 1% glutaraldehyde solution,washed with PBS and then stained withsafranin. 10 ml of stained blood cells wasinjected into the internal iliac artery of thecadaver. The stained blood cells injected intothe internal iliac artery differentiated theinternal pudendal arteries from the pudendalnerve and the inferior rectal artery from theinferior rectal nerve. The preparation ofstained blood cells and their application couldbe used by all medical and dental students tofacilitate learning of the functional arteries (AU)

RESUMEN

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