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Exosomes derived from adipose tissues accelerate fibroblasts and keratinocytes proliferation and cutaneous wound healing via miR-92a/Hippo-YAP axis
Shao, Zifei; Xu, Jinghao; Zhou, Yuxi; Wang, Yujing; Li, Yiyang; Zhao, Jianping; Li, Kun.
Afiliação
  • Shao, Zifei; Xiangya Stomatological Hospital & School of Stomatology. Central South University. Changsha. China
  • Xu, Jinghao; Xiangya Stomatological Hospital & School of Stomatology. Central South University. Changsha. China
  • Zhou, Yuxi; Xiangya Stomatological Hospital & School of Stomatology. Central South University. Changsha. China
  • Wang, Yujing; Xiangya Stomatological Hospital & School of Stomatology. Central South University. Changsha. China
  • Li, Yiyang; Xiangya Stomatological Hospital & School of Stomatology. Central South University. Changsha. China
  • Zhao, Jianping; Dushu Lake Hospital Affiliated to Soochow University. Department of Stomatology. Suzhou. China
  • Li, Kun; Xiangya Stomatological Hospital & School of Stomatology. Central South University. Changsha. China
J. physiol. biochem ; 80(1): 189-204, Feb. 2024. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-EMG-576
Biblioteca responsável: ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Delayed wound healing is an urgent clinical issue. Cellular communication involving exosome-borne cargo such as miRNA is a critical mechanism involved in wound healing. This study isolated and identified human adipose tissue-derived exosomes (Exo-ATs). The specific effects of Exo-ATs on keratinocytes and fibroblasts were examined. Enriched miRNAs in Exo-ATs were analyzed, and miR-92a-3p was selected. The transfer of Exo-ATs-derived miR-92a-3p to keratinocytes and fibroblasts was verified. miR-92a-3p binding to LATS2 was examined and the dynamic effects of the miR-92a-3p/LATS2 axis were investigated. In a dorsal skin wound model, the in vivo effects of Exo-ATs on wound healing were examined. Exo-AT incubation increased keratinocytes and fibroblast proliferation, migration, and extracellular matrix (ECM) accumulation. miR-92a-3p, enriched in Exo-ATs, could be transferred to keratinocytes and fibroblasts, resulting in enhanced proliferation, migration, and ECM accumulation. Large tumor suppressor kinase 2 (LATS2) was a direct target of miR-92a-3p. miR-92a-3p inhibitor effects on keratinocytes and fibroblasts could be partially reversed by LATS2 knockdown. In a dorsal skin wound model, Exo-ATs accelerated wound healing through enhanced cell proliferation, collagen deposition, re-epithelialization, and YAP/TAZ activation. In conclusion, Exo-ATs improve skin wound healing by promoting keratinocyte and fibroblast migration and proliferation and collagen production by fibroblast, which could be partially eliminated by miR-92a inhibition through its downstream target LATS2 and the YAP/TAZ signaling. (AU)
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Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Cicatrização / Proliferação de Células / Exossomos / Neoplasias Pulmonares / Síndromes Paraneoplásicas / Pneumonia / Recidiva / Deficiência de Vitamina D / Transplante de Rim / Segunda Neoplasia Primária Tipo de estudo: Ensaio clínico controlado / Estudo observacional / Fatores de risco Limite: Humanos / Masculino / Adulto / Idoso / Feminino País/Região como assunto: América do Sul / Brasil / Europa Idioma: Inglês / Espanhol Revista: Arch. bronconeumol. (Ed. impr.) / J. physiol. biochem / An. pediatr. (2003. Ed. impr.) / Nutr. hosp Ano de publicação: 2023 / 2022 / 2024 Tipo de documento: Artigo Instituição/País de afiliação: Dushu Lake Hospital Affiliated to Soochow University/China / Xiangya Stomatological Hospital & School of Stomatology/China / Centro Hospitalario Universitário do Algarve – Hospital de Faro/Portugal / Hospital Clínico Universitario de Valladolid/Spain / Hospital Universitario Puerta de Hierro-Majadahonda/España / Puerta del Mar University Hospital/Spain / Universidade Federal do Rio Grande do Norte/Brazil / Virgen Macarena University Hospital/Spain / Virgen de las Nieves University Hospital/Spain / Virgen del Rocío University Hospital/Spain
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Texto completo: Disponível Coleções: Bases de dados nacionais / Espanha Base de dados: IBECS Assunto principal: Cicatrização / Proliferação de Células / Exossomos / Neoplasias Pulmonares / Síndromes Paraneoplásicas / Pneumonia / Recidiva / Deficiência de Vitamina D / Transplante de Rim / Segunda Neoplasia Primária Tipo de estudo: Ensaio clínico controlado / Estudo observacional / Fatores de risco Limite: Humanos / Masculino / Adulto / Idoso / Feminino País/Região como assunto: América do Sul / Brasil / Europa Idioma: Inglês / Espanhol Revista: Arch. bronconeumol. (Ed. impr.) / J. physiol. biochem / An. pediatr. (2003. Ed. impr.) / Nutr. hosp Ano de publicação: 2023 / 2022 / 2024 Tipo de documento: Artigo Instituição/País de afiliação: Dushu Lake Hospital Affiliated to Soochow University/China / Xiangya Stomatological Hospital & School of Stomatology/China / Centro Hospitalario Universitário do Algarve – Hospital de Faro/Portugal / Hospital Clínico Universitario de Valladolid/Spain / Hospital Universitario Puerta de Hierro-Majadahonda/España / Puerta del Mar University Hospital/Spain / Universidade Federal do Rio Grande do Norte/Brazil / Virgen Macarena University Hospital/Spain / Virgen de las Nieves University Hospital/Spain / Virgen del Rocío University Hospital/Spain
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