Comparative genomic analysis of Halomonas campaniensis wild-type and ultraviolet radiation-mutated strains reveal genomic differences associated with increased ectoine production / El análisis genómico comparativo de cepas mutadas por radiación ultravioleta y de tipo salvaje de Halomonas campaniensis revela diferencias genómicas asociadas con una mayor producción de ectoína
Int. microbiol
; 26(4): 1009-1020, Nov. 2023. ilus, graf
Artigo
em Inglês
| IBECS
| ID: ibc-227488
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Ectoine is a natural amino acid derivative and one of the most widely used compatible solutes produced by Halomonas species that affects both cellular growth and osmotic equilibrium. The positive effects of UV mutagenesis on both biomass and ectoine content production in ectoine-producing strains have yet to be reported. In this study, the wild-type H. campaniensis strain XH26 (CCTCCM2019776) was subjected to UV mutagenesis to increase ectoine production. Eight rounds of mutagenesis were used to generate mutated XH26 strains with different UV-irradiation exposure times. Ectoine extract concentrations were then evaluated among all strains using high-performance liquid chromatography analysis, alongside whole genome sequencing with the PacBio RS II platform and comparison of the wild-type strain XH26 and the mutant strain G8-52 genomes. The mutant strain G8-52 (CCTCCM2019777) exhibited the highest cell growth rate and ectoine yields among mutated strains in comparison with strain XH26. Further, ectoine levels in the aforementioned strain significantly increased to 1.51 ± 0.01 g L−1 (0.65 g g−1 of cell dry weight), representing a twofold increase compared to wild-type cells (0.51 ± 0.01 g L−1) when grown in culture medium for ectoine accumulation. Concomitantly, electron microscopy revealed that mutated strain G8-52 cells were obviously shorter than wild-type strain XH26 cells. Moreover, strain G8-52 produced a relatively stable ectoine yield (1.50 g L−1) after 40 days of continuous subculture. Comparative genomics analysis suggested that strain XH26 harbored 24 mutations, including 10 nucleotide insertions, 10 nucleotide deletions, and unique single nucleotide polymorphisms. Notably, the genes orf00723 and orf02403 (lipA) of the wild-type strain mutated to davT and gabD in strain G8-52 that encoded for 4-aminobutyrate-2-oxoglutarate transaminase and NAD-dependent succinate-semialdehyde dehydrogenase, respectively. Consequently, these genes may be involved in increased ectoine yields. These results suggest that continuous multiple rounds of UV mutation represent a successful strategy for increasing ectoine production, and that the mutant strain G8-52 is suitable for large-scale fermentation applications.(AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Pandemias
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Betacoronavirus
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Enfermeiras e Enfermeiros
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Exame Físico
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Diagnóstico Pré-Natal
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Opinião Pública
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Sialorreia
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Medicina Esportiva
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Estudantes de Enfermagem
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Cirurgia Torácica
Limite:
Humanos
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Feminino
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Masculino
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Criança
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Adolescente
País/Região como assunto:
Europa
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América do Sul
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Ásia
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Chile
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México
Idioma:
Espanhol
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Inglês
Revista:
Metas enferm
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An. psicol
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Arch. med. deporte
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Asclepio
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Aten. prim. (Barc., Ed. impr.)
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Enferm. clín. (Ed. impr.)
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Int. microbiol
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Med. clín (Ed. impr.)
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Radiología (Madr., Ed. impr.)
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Rev. Fund. Educ. Méd. (Ed. impr.)
Ano de publicação:
2022
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2023
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2021
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2024
Tipo de documento:
Artigo
Instituição/País de afiliação:
Centre de Salut Joan Mirambell i Folch, Institut Català de la Salut (ICS)/España
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Christian University/Thailand
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Fisiorespi/España
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Fundación Jiménez Díaz University Hospital/Spain
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Health Affairs at Ministry of Health Saudi Arabia/Saudi Arabia
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Hospital Clínic de Barcelona/España
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Hospital HM Puerta del Su/España
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Hospital Infantil Universitario Niño Jesús/España
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Hospital Universitario 12 de Octubre/España
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Hospital Universitario Fundación Jiménez Díaz/España