Efectos de la radiación ultravioleta A y B sobre linfocitos humanos / Effects of A and B ultraviolet irradiation over human lymphocutes

ABSTRACT

Over the last decade, agreter decrease in the ozone level has occurred in the Southern Hemisphere. For each 1 per cent decrease in this level, a 2 per cent increase in biologically effective radiation occurs. Aiming to evaluate the biological effect produced by UV radiation, 10 blood samples coming from patients consulting for reproductive problems, were irradiated with visible and ultraviolet radiation A (treatment A) and visible and ultraviolet radiation B (treatment B) during 1 to 5 minutes. This dosage is comparable to the radiation received in Santiago at 13:00 h in a summer day. After irradiation, lymphocytes were cultured during 72 h and the number of altered metaphases was quantified. There was a significant increase in chromosomal alterations with treatment A (2.61, 2.43, 4.53 and 3.53 at 1, 2, 3 and 5 min respectively) and treatment B (3.06, 3.81, 3.3, 5.51, at 1, 2, 3 and 5 min respectively) compared with non-irradiated controls (0.8 and 0.72). There was a reduction in mitotic indices in irradiated cells. It is concluded that both types of UV radiation (A and B) produce chromosomal alternations in vitro, even using lower doses than those received during summer in the central region of Chile