Purification and properties of storage proteins (vicilins) from cowpea (Vigna unguiculata) seeds which are susceptible or resistant to the bruchid beethe Callosobruchus maculatus
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;28(2): 183-90, Feb. 1995. ilus, tab, graf
Article
em En
| LILACS
| ID: lil-154262
Biblioteca responsável:
BR1.1
RESUMO
Vicilins (7S storage proteins) from seeds of cowpea (Vigna unguiculata) cultivars which are susceptible or resistant to the bruchil veetle C. maculatus were purified by size-exclusion and ion-exchange chromatography. The vicilins were partially characterized by polyacrylamide gel electrophoresis under both denaturing and non-denaturing conditions, by Western blotting and by amino acid analysis. The variant vicilins from C. maculatus-resistant seeds do not differ appreciably from vicilins from susceptible seeds by these criteria except that they are more strongly bound to DEAE-Sepharose, suggesting differences in charge between the various molecules
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Proteínas de Plantas
/
Sementes
/
Besouros
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1995
Tipo de documento:
Article
País de publicação:
Brasil