Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
Braz. j. med. biol. res
; 29(4): 489-94, Apr. 1996. graf
Artigo
em Inglês
| LILACS
| ID: lil-163891
Biblioteca responsável:
BR1.1
RESUMO
Anticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CLPC. The sera dilution used here was 17500. IgG (5-20 mug/ml) from the same sera, not containing beta2GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.
Buscar no Google
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Cardiolipinas
/
Sífilis
/
Anticorpos Anticardiolipina
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1996
Tipo de documento:
Artigo