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Serine/threonine protein phosphatases and a protein phosphatase 1 inhibitor from Neurospora crassa
Braz. j. med. biol. res ; 29(5): 599-604, May 1996. graf
Artigo em Inglês | LILACS | ID: lil-182542
Biblioteca responsável: BR1.1
ABSTRACT
The major spontaneously active serine/threonine (Ser/Thr) protein phosphatase activities in N. crassa wild type (FGSC 424) were type 1 (PP1), type-2A (PP2A) and type-2C (PP2C). PP1 and PP2C predominantly dephosphorylated phosphorylase a and casein, respectively. PP2A acted on both substrates, but was two-fold more active against casein. PPI activity was inhibited by protamine, heparin, okadaic acid (IC50 50 nM) and mammalian inhibitor- 1 (lC50 2 nM). On the other hand, PP2A activity was inhibited by much lower concentrations of okadaic acid (IC50 0.2 nM) and also by protamine, but not by heparin or inhibitor-l. About 80 per cent of total PP1 activity was associated with the particulate fraction and could be partially extracted with 0.5 M NaCl. Seventy and ninety percent of PP2A and PP2C activities, respectively, were found in the soluble fraction. In addition we have partially purified an acid and thermostable PP1 inhibitor which effectively inhibits both N. crassa and mammalian PP1.
Assuntos
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Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Serina / Treonina / Fosfoproteínas Fosfatases / Éteres Cíclicos / Neurospora crassa Limite: Animais Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 1996 Tipo de documento: Artigo
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Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Serina / Treonina / Fosfoproteínas Fosfatases / Éteres Cíclicos / Neurospora crassa Limite: Animais Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 1996 Tipo de documento: Artigo
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