Biological parameters and molecular markers of clone CL brener - the reference organism of the Trypanosoma cruzi genome project
Mem. Inst. Oswaldo Cruz
; 92(6): 811-4, Nov.-Dec. 1997. ilus, tab
Article
em En
| LILACS
| ID: lil-197221
Biblioteca responsável:
BR15.1
ABSTRACT
Clone CL Brener is the reference organism used in the Trypanosma cruzi Genome Project. Some biological paramenters of CL Brener were determined (a) the doubling time of epimastigote forms cultured in liver infusion-tryptose (LIT) medium at 28ºC is 58ñ13 hr; (b) differentiation of epimastigotes to metacyclic trypomastigotes is obtained by incubation in LIT-20 per cent Grace's medium; (c) trypomastigotes infect mammalian cultured cells and perform the complete intracellular cycle at 33 and 37ºC; (c) blood forms are highly infective to mice; (e) blood forms are susceptible to nifurtimox and benznidazole. The molecular typing of CL Brener has been determined (a) isoenzymatic profiles are characteristic of zymodeme ZB; (b) PCR amplification of a 24 alpha ribosomal RNA sequence indicates it belongs to T. cruzi lineage 1; (c) schizodeme, randomly amplified polymorphic DNA (RAPD) and DNA fingerprinting analyses were performed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Trypanosoma cruzi
/
Células Clonais
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
/
Congress and conference