The role of natural killer cells in the early period of infection in murine cutaneous leishmaniasis
Braz. j. med. biol. res
; 32(3): 323-5, Mar. 1999.
Article
em En
| LILACS
| ID: lil-230460
Biblioteca responsável:
BR1.1
ABSTRACT
In order to study the role of natural killer (NK) cells during the early period of Leishmania infection, BALB/c mice were selectively and permanently depleted of NK cells by injection with 90Sr and subsequently infected with Leishmania (Leishmania) amazonensis (HSJD-1 strain). 90Sr is known to selectively deplete NK cells, leaving an intact T- and B-cell compartment and preserving the ability to produce both interferon alpha and IL-2. This method of depletion has advantages when compared with depletion using anti-NK cell monoclonal antibodies because the effect is permanent and neither activates complement nor provokes massive cell death. In the present study, after one month of treatment with 90Sr, the depletion of NK cells was shown by a more than ten-fold reduction in the cytotoxic activity of these cells 2 x 106 spleen cells from NK-depleted animals were required to reach the same specific lysis of target cells effected by 0.15 x 106 spleen cells from normal control animals. The histopathology of the skin lesion at 7 days after Leishmania infection showed more parasites in the NK cell-depleted group. This observation further strengthens a direct role of NK cells during the early period of Leishmania infection
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Radioisótopos de Estrôncio
/
Células Matadoras Naturais
/
Leishmaniose Cutânea
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1999
Tipo de documento:
Article
/
Congress and conference
País de afiliação:
Brasil
País de publicação:
Brasil