Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced by "Bacillus" sp
Rev. microbiol
; 30(4): 299-303, out.-dez. 1999. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-286783
Biblioteca responsável:
BR32.1
RESUMO
One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use of citrus pectin as the sole carbon source. 102 were positive for pectinase depolymerization in assay plates as evidenced by clear hydrolization halos. Among them, 30(per cent) presented considerable pectinolytic activity. The cultivation of these strains by submerged and semi-solid fermentation for polygalacturose production indicated that five strains of "Bacillus" sp. produced high quantities of the enzyme. The physico-chemical characteristics, such as optimum pH of 6.0-7.0, optimum temperatures between 45ºC and 55ºC, stability at temperatures above 40ºC and in neutral and alkaline pH, were determined
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Poligalacturonase
/
Bacillus
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Rev. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
1999
Tipo de documento:
Artigo