Resistance of multicellular aggregates to pharmorubicin observed in human hepatocarcinoma cells
Braz. j. med. biol. res
; 35(2): 255-260, Feb. 2002. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-303556
Biblioteca responsável:
BR1.1
RESUMO
The objective of the present study was to investigate the multicellular resistance of human hepatocarcinoma cells BEL-7402 to pharmorubicin. Cells (1 x 10(4)) and 200 microcarrier Cytodex-3 beads were seeded onto a 24-well plate and cultured in RPMI 1640 medium. After the formation of multicellular aggregates, morphology and cell viability were analyzed by scanning electron microscopy, transmission electron microscopy and flow cytometry, respectively. The IC50 was determined by flow cytometry and MTT assay after the cells cultured in aggregates and monolayers were treated with pharmorubicin. The culture products exhibited structural characteristics somewhat similar to those of trabecular hepatocarcinoma in vivo. Among the microcarriers, cells were organized into several layers. Intercellular spaces were 0.5-2.0 æm wide and filled with many microvilli. The percent of viable cells was 87 percent. The cells cultured as multicellular aggregates were resistant to pharmorubicin with IC50 4.5-fold and 7.7-fold that of monolayer culture as determined by flow cytometry and MTT assay, respectively. This three-dimensional culture model may be used to investigate the mechanisms of multicellular drug resistance of hepatocarcinoma and to screen new anticancer drugs
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Epirubicina
/
Carcinoma Hepatocelular
/
Esferoides Celulares
/
Neoplasias Hepáticas
/
Antibióticos Antineoplásicos
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Chinese Academy of Sciences/CN