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Normal erythrocyte calpain I activity on membrane proteins under near-physiological conditions in patients with essential hypertension
Medeiros, Tereza Maria Dantas de; Ortega, Katia Coelho; Mion Júnior, Décio; Nonoyama, Kimiyo; Barretto, Orlando Cesar de Oliveira.
Afiliação
  • Medeiros, Tereza Maria Dantas de; Universidade Federal do Rio Grande do Norte. Clinical Chemistry and Toxicology Department. Natal. BR
  • Ortega, Katia Coelho; Universidade de Säo Paulo. Faculty of Medicine. Hospital das Clínicas. Hypertension Division. Säo Paulo. BR
  • Mion Júnior, Décio; Universidade de Säo Paulo. Faculty of Medicine. Hospital das Clínicas. Hypertension Division. Säo Paulo. BR
  • Nonoyama, Kimiyo; Instituto Adolfo Lutz. Clinical Pathology Department. Hematology Division. Säo Paulo. BR
  • Barretto, Orlando Cesar de Oliveira; Universidade de Säo Paulo. Faculty of Medicine. Psychiatry Department. LIM-23. Säo Paulo. BR
Säo Paulo med. j ; 120(1): 05-08, jan. 2002. ilus, tab
Artigo em Inglês | LILACS | ID: lil-303889
Biblioteca responsável: BR1.1
RESUMO
CONTEXT It has been reported that the equilibrium between the erythrocyte protease calpain I and its physiological inhibitor calpastatin is disrupted in patients with essential hypertension.

OBJECTIVE:

To investigate the activity of non-purified calpain I in hemolysates against the erythrocytic membrane proteins, rather than against other substrates.

DESIGN:

Evaluation of calpain I red cell activity upon its own physiological substrates in hypertensive patients, in a near-physiological environment.

SETTING:

LIM-23 and LIM-40 of Hospital das Clinicas of the Faculty of Medicine of USP. SAMPLE Patients with moderate primary hypertension over 21 years of age who were given amlodipine (n10) and captopril (n10) for 8 weeks, plus normal controls (n10). MAIN MEASUREMENTS Red cell membrane proteins were incubated with and without protease inhibitors and with and without calcium chloride and underwent polyacrylamide gel electrophoresis.

RESULTS:

Digestion of bands 2.1 and 4.1 was observed, indicating calpain I acitivity. No statistical differences regarding bands 2.1 and 4.1 were observed before treatment, between the controls and the hypertensive patients, either in ghosts prepared without calcium or with increasing concentrations of calcium. Nor were statistical differences observed after treatment, between the controls and the patients treated with amlodipine and captopril, or between the patients before and after treatment with both drugs.

CONCLUSION:

The final activity of non-purified calpain I upon its own physiological substrate, which was the approach utilized in this study, may more adequately reflect what happens in red cells. Under such conditions no imbalance favoring calpain I activity increase was observed. The protective factor provided by calpastatin against calpain I activity may diminish under hypertension
Assuntos
Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Calpaína / Membrana Eritrocítica / Hipertensão / Proteínas de Membrana Limite: Adulto / Humanos Idioma: Inglês Revista: Säo Paulo med. j Assunto da revista: Medicina Ano de publicação: 2002 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Instituto Adolfo Lutz/BR / Universidade Federal do Rio Grande do Norte/BR / Universidade de Säo Paulo/BR

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Calpaína / Membrana Eritrocítica / Hipertensão / Proteínas de Membrana Limite: Adulto / Humanos Idioma: Inglês Revista: Säo Paulo med. j Assunto da revista: Medicina Ano de publicação: 2002 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Instituto Adolfo Lutz/BR / Universidade Federal do Rio Grande do Norte/BR / Universidade de Säo Paulo/BR
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