Nitric oxide, cholesterol oxides and endothelium-dependent vasodilation in plasma of patients with essential hypertension
Braz. j. med. biol. res
; 35(11): 1301-1309, Nov. 2002. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-326262
Biblioteca responsável:
BR1.1
ABSTRACT
The objective of the present study was to identify disturbances of nitric oxide radical (ANO) metabolism and the formation of cholesterol oxidation products in human essential hypertension. The concentrations ofANO derivatives (nitrite, nitrate, S-nitrosothiols and nitrotyrosine), water and lipid-soluble antioxidants and cholesterol oxides were measured in plasma of 11 patients with mild essential hypertension (H 57.8 ± 9.7 years; blood pressure, 148.3 ± 24.8/90.8 ± 10.2 mmHg) and in 11 healthy subjects (N 48.4 ± 7.0 years; blood pressure, 119.4 ± 9.4/75.0 ± 8.0 mmHg).Nitrite, nitrate and S-nitrosothiols were measured by chemiluminescence and nitrotyrosine was determined by ELISA. Antioxidants were determined by reverse-phase HPLC and cholesterol oxides by gas chromatography. Hypertensive patients had reduced endothelium-dependent vasodilation in response to reactive hyperemia (H 9.3 and N 15.1 percent increase of diameter 90 s after hyperemia), and lower levels of ascorbate (H 29.2 ± 26.0, N 54.2 ± 24.9 æM), urate (H 108.5 ± 18.9, N 156.4 ± 26.3 æM), ß-carotene (H 1.1 ± 0.8, N 2.5 ± 1.2 nmol/mg cholesterol), and lycopene (H 0.4 ± 0.2, N 0.7 ± 0.2 nmol/mg cholesterol), in plasma, compared to normotensive subjects. The content of 7-ketocholesterol, 5alpha-cholestane-3ß,5,6ß-triol and 5,6alpha-epoxy-5alpha-cholestan-3alpha-ol in LDL, and the concentration of endothelin-1 (H 0.9 ± 0.2, N 0.7 ± 0.1 ng/ml) in plasma were increased in hypertensive patients. No differences were found for ANO derivatives between groups. These data suggest that an increase in cholesterol oxidation is associated with endothelium dysfunction in essential hypertension and oxidative stress, although ANO metabolite levels in plasma are not modified in the presence of elevated cholesterol oxides
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Endotélio Vascular
/
Peroxidação de Lipídeos
/
Estresse Oxidativo
/
Hipertensão
/
Óxido Nítrico
Tipo de estudo:
Estudo observacional
/
Fatores de risco
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2002
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
/
Estados Unidos
/
Uruguai
Instituição/País de afiliação:
Universidad de la Republica/UY
/
Universidade Federal de Säo Paulo/BR
/
Universidade de Säo Paulo/BR
/
University of Southern California/US